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Therefore, antibody probes of high titer and high affinity are now available for the study of this important nuclear enzyme.<\/jats:p>","DOI":"10.1126\/science.3003910","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:52Z","timestamp":1160080132000},"page":"737-740","source":"Crossref","is-referenced-by-count":241,"title":["High Titers of Autoantibodies to Topoisomerase I (Scl-70) in Sera from Scleroderma Patients"],"prefix":"10.1126","volume":"231","author":[{"given":"J. H.","family":"Shero","sequence":"first","affiliation":[{"name":"Department of Cell\rBiology and Anatomy, Johns Hopkins School of Medicine,\rBaltimore, MD 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Bordwell","sequence":"additional","affiliation":[{"name":"Division of Rheumatic\rDiseases, Department of Medicine, University of Connecticut\rHealth Sciences Center, Farmington 06032."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. F.","family":"Rothfield","sequence":"additional","affiliation":[{"name":"Division of Rheumatic\rDiseases, Department of Medicine, University of Connecticut\rHealth Sciences Center, Farmington 06032."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. C.","family":"Earnshaw","sequence":"additional","affiliation":[{"name":"Department of Cell\rBiology and Anatomy, Johns Hopkins School of Medicine,\rBaltimore, MD 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"2115","volume":"82","year":"1985","unstructured":"CHAMBERS, J.C., ISOLATION AND ANALYSIS OF CDNA CLONES EXPRESSING HUMAN LUPUS LA ANTIGEN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 2115 (1985).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"4638","DOI":"10.1021\/bi00666a014","volume":"15","year":"1976","unstructured":"CHAMPOUX, J.J., PURIFICATION AND CHARACTERIZATION OF DNA UNTWISTING ENZYME FROM RAT-LIVER, BIOCHEMISTRY 15: 4638 (1976).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/S0021-9258(19)75212-0","volume":"252","year":"1977","unstructured":"CLEVELAND, D.W., PEPTIDE MAPPING BY LIMITED PROTEOLYSIS IN SODIUM DODECYL-SULFATE AND ANALYSIS BY GEL-ELECTROPHORESIS, JOURNAL OF BIOLOGICAL CHEMISTRY 252: 1102 (1977).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"10514","DOI":"10.1016\/S0021-9258(19)86738-8","volume":"254","year":"1979","unstructured":"DOUVAS, A.S., IDENTIFICATION OF A NUCLEAR-PROTEIN (SCL-70) AS A UNIQUE TARGET OF HUMAN ANTI-NUCLEAR ANTIBODIES IN SCLERODERMA, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 10514 (1979).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/BF00328227","volume":"91","year":"1985","unstructured":"EARNSHAW, W.C., IDENTIFICATION OF A FAMILY OF HUMAN CENTROMERE PROTEINS USING AUTOIMMUNE SERA FROM PATIENTS WITH SCLERODERMA, CHROMOSOMA 91: 313 (1985).","journal-title":"CHROMOSOMA"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1083\/jcb.98.1.352","volume":"98","year":"1984","unstructured":"EARNSHAW, W.C., THE KINETOCHORE IS PART OF THE METAPHASE CHROMOSOME SCAFFOLD, JOURNAL OF CELL BIOLOGY 98: 352 (1984).","journal-title":"JOURNAL OF CELL BIOLOGY"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1083\/jcb.100.5.1706","volume":"100","year":"1985","unstructured":"EARNSHAW, W.C., TOPOISOMERASE-II IS A STRUCTURAL COMPONENT OF MITOTIC CHROMOSOME SCAFFOLDS, JOURNAL OF CELL BIOLOGY 100: 1706 (1985).","journal-title":"JOURNAL OF CELL BIOLOGY"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1083\/jcb.100.5.1716","volume":"100","year":"1985","unstructured":"EARNSHAW, W.C., LOCALIZATION OF TOPOISOMERASE-II IN MITOTIC CHROMOSOMES, JOURNAL OF CELL BIOLOGY 100: 1716 (1985).","journal-title":"JOURNAL OF CELL BIOLOGY"},{"key":"e_1_2_1_9_1","unstructured":"EARNSHAW W.C. unpublished data."},{"key":"e_1_2_1_10_1","first-page":"7410","volume":"80","year":"1983","unstructured":"HARDIN, J.A., ANTIBODIES TO HISTONES IN SYSTEMIC LUPUS-ERYTHEMATOSUS - 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