{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:46:43Z","timestamp":1791352003392,"version":"4.3.1"},"reference-count":29,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4761","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1986,7,18]]},"abstract":"<jats:p>A human complementary DNA whose protein product is considered to be the major component of scrapie-associated fibrils in Creutzfeldt-Jakob disease, kuru, and Gerstmann-Straussler syndrome has been identified and characterized. The extensive homology of this gene sequence to the hamster PrP 27- to 30-kilodalton prion protein complementary DNA clone, and its existence as a single copy in the human genome, leads to the conclusion that this is the human prion gene. This human prion gene has been mapped to human chromosome 20, negating a direct link between the prion protein and Down's syndrome or the amyloid of Alzheimer's disease.<\/jats:p>","DOI":"10.1126\/science.3014653","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:52Z","timestamp":1160080132000},"page":"364-367","source":"Crossref","is-referenced-by-count":114,"title":["Human Prion Protein cDNA: Molecular Cloning, Chromosomal Mapping, and Biological Implications"],"prefix":"10.1126","volume":"233","author":[{"given":"Yu-Cheng","family":"Liao","sequence":"first","affiliation":[{"name":"Department of Pathology, University of California, San Francisco, CA 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger V.","family":"Lebo","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary A.","family":"Clawson","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California, San Francisco, CA 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A.","family":"Smuckler","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California, San Francisco, CA 94143."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/310418a0","volume":"310","year":"1984","unstructured":"BENDHEIM, P.E., ANTIBODIES TO A SCRAPIE PRION PROTEIN, NATURE 310: 418 (1984).","journal-title":"NATURE"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"5898","DOI":"10.1021\/bi00320a002","volume":"23","year":"1984","unstructured":"BOLTON, D.C., MOLECULAR CHARACTERISTICS OF THE MAJOR SCRAPIE PRION PROTEIN, BIOCHEMISTRY 23: 5898 (1984).","journal-title":"BIOCHEMISTRY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6815801"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1002\/j.1460-2075.1985.tb03931.x","volume":"4","year":"1985","unstructured":"BRAIG, H.R., SCRAPIE - CONCEPT OF A VIRUS-INDUCED AMYLOIDOSIS OF THE BRAIN, EMBO JOURNAL 4: 2309 (1985).","journal-title":"EMBO JOURNAL"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.1365-2990.1976.tb00521.x","volume":"2","year":"1976","unstructured":"BRUCE, M.E., CEREBRAL AMYLOIDOSIS IN SCRAPIE IN MOUSE - EFFECT OF AGENT STRAIN AND MOUSE GENOTYPE, NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY 2: 471 (1976).","journal-title":"NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/315331a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(85)90053-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/306476a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.142303"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1126\/science.2933807","volume":"230","year":"1985","unstructured":"KOLATA, G, DOWN SYNDROME ALZHEIMERS LINKED, SCIENCE 230: 1152 (1985).","journal-title":"SCIENCE"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S0076-6879(87)51025-4","volume":"151","year":"1987","unstructured":"Lebo, R. V., Methods in Enzymology 151: 292 (1987).","journal-title":"Methods in Enzymology"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6587566"},{"key":"e_1_2_1_14_1","unstructured":"LEBO R.V. unpublished data."},{"key":"e_1_2_1_15_1","first-page":"4263","volume":"82","year":"1985","unstructured":"MANUELIDIS, L, SPECIFIC PROTEINS ASSOCIATED WITH CREUTZFELDT-JAKOB DISEASE AND SCRAPIE SHARE ANTIGENIC AND CARBOHYDRATE DETERMINANTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 4263 (1985).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/0092-8674(84)90456-2","volume":"39","year":"1984","unstructured":"MARCHUK, D, REMARKABLE CONSERVATION OF STRUCTURE AMONG INTERMEDIATE FILAMENT GENES, CELL 39: 491 (1984).","journal-title":"CELL"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0092-8674(83)90207-6","volume":"35","year":"1983","unstructured":"MCKINLEY, M.P., A PROTEASE-RESISTANT PROTEIN IS A STRUCTURAL COMPONENT OF THE SCRAPIE PRION, CELL 35: 57 (1983).","journal-title":"CELL"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/BF00691333","volume":"54","year":"1981","unstructured":"MERZ, P.A., ABNORMAL FIBRILS FROM SCRAPIE-INFECTED BRAIN, ACTA NEUROPATHOLOGICA 54: 63 (1981).","journal-title":"ACTA NEUROPATHOLOGICA"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1038\/306474a0","volume":"306","year":"1983","unstructured":"MERZ, P.A., SCRAPIE-ASSOCIATED FIBRILS IN CREUTZFELDT-JAKOB DISEASE, NATURE 306: 474 (1983).","journal-title":"NATURE"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6377496"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb03808.x"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90333-2"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1002\/ana.410110406","volume":"11","year":"1982","unstructured":"PRUSINER, S.B., MEASUREMENT OF THE SCRAPIE AGENT USING AN INCUBATION-TIME INTERVAL ASSAY, ANNALS OF NEUROLOGY 11: 353 (1982).","journal-title":"ANNALS OF NEUROLOGY"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90168-X"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0092-8674(84)90533-6","volume":"38","year":"1984","unstructured":"PRUSINER, S.B., PURIFICATION AND STRUCTURAL STUDIES OF A MAJOR SCRAPIE PRION PROTEIN, CELL 38: 127 (1984).","journal-title":"CELL"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6801762"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1177\/10.3.355","volume":"10","year":"1962","unstructured":"PUCHTLER, H, ON BINDING OF CONGO RED BY AMYLOID, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 10: 355 (1962).","journal-title":"JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_1_29_1","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1093\/nar\/14.5.2035","volume":"14","year":"1986","unstructured":"WESTAWAY, D, CONSERVATION OF THE CELLULAR GENE ENCODING THE SCRAPIE PRION PROTEIN, NUCLEIC ACIDS RESEARCH 14: 2035 (1986).","journal-title":"NUCLEIC ACIDS RESEARCH"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.3014653","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T15:41:52Z","timestamp":1705160512000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.3014653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,7,18]]},"references-count":29,"journal-issue":{"issue":"4761","published-print":{"date-parts":[[1986,7,18]]}},"alternative-id":["10.1126\/science.3014653"],"URL":"https:\/\/doi.org\/10.1126\/science.3014653","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,7,18]]}}}