{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:18:43Z","timestamp":1790745523252,"version":"4.1.0"},"reference-count":23,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4744","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1986,3,21]]},"abstract":"<jats:p>\n            Structural data are now available for comparing a penicillin target enzyme, the D-alanyl-D-alanine-peptidase from\n            <jats:italic>Streptomyces<\/jats:italic>\n            R61, with a penicillin-hydrolyzing enzyme, the \u03b2-lactamase from\n            <jats:italic>Bacillus licheniformis<\/jats:italic>\n            749\/C. Although the two enzymes have distinct catalytic properties and lack relatedness in their overall amino acid sequences except near the active-site serine, the significant similarity found by x-ray crystallography in the spatial arrangement of the elements of secondary structure provides strong support for earlier hypotheses that \u03b2-lactamases arose from penicillin-sensitive D-alanyl-D-alanine-peptidases involved in bacterial wall peptidoglycan metabolism.\n          <\/jats:p>","DOI":"10.1126\/science.3082007","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:52Z","timestamp":1160080132000},"page":"1429-1431","source":"Crossref","is-referenced-by-count":168,"title":["On the Origin of Bacterial Resistance to Penicillin: Comparison of a \u03b2-Lactamase and a Penicillin Target"],"prefix":"10.1126","volume":"231","author":[{"given":"J. A.","family":"Kelly","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology and Institute of Materials Science, University of Connecticut, Storrs, 06268."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Dideberg","sequence":"additional","affiliation":[{"name":"Service de Cristallographie, Institut de Physique, B5, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Charlier","sequence":"additional","affiliation":[{"name":"Service de Cristallographie, Institut de Physique, B5, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Wery","sequence":"additional","affiliation":[{"name":"Service de Cristallographie, Institut de Physique, B5, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Libert","sequence":"additional","affiliation":[{"name":"Service de Cristallographie, Institut de Physique, B5, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. C.","family":"Moews","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology and Institute of Materials Science, University of Connecticut, Storrs, 06268."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R","family":"Knox","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology and Institute of Materials Science, University of Connecticut, Storrs, 06268."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Duez","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Institut de Chimie, B6, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl.","family":"Fraipont","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Institut de Chimie, B6, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Joris","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Institut de Chimie, B6, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Dusart","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Institut de Chimie, B6, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Fr\u00e8re","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Institut de Chimie, B6, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Ghuysen","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Institut de Chimie, B6, Universit\u00e9 de Li\u00e8ge, B-4000 Sart Tilman, Li\u00e8ge, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"321","volume":"289","year":"1980","unstructured":"AMBLER, R.P., THE STRUCTURE OF BETA-LACTAMASES, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 289: 321 (1980).","journal-title":"PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/0022-2836(78)90430-8","volume":"120","year":"1978","unstructured":"ASCHAFFENBURG, R, PRELIMINARY CRYSTALLOGRAPHIC DATA FOR BETA-LACTAMASE-I FROM BACILLUS-CEREUS-569, JOURNAL OF MOLECULAR BIOLOGY 120: 447 (1978).","journal-title":"JOURNAL OF MOLECULAR BIOLOGY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1042\/bj2290791","volume":"229","year":"1985","unstructured":"BICKNELL, R, THE PRODUCTION AND MOLECULAR-PROPERTIES OF THE ZINC BETA-LACTAMASE OF PSEUDOMONAS-MALTOPHILIA IID-1275, BIOCHEMICAL JOURNAL 229: 791 (1985).","journal-title":"BIOCHEMICAL JOURNAL"},{"key":"e_1_2_1_4_1","first-page":"341","volume":"3","year":"1983","unstructured":"CARTWRIGHT, S.J., BETA-LACTAMASE INHIBITORS, MEDICINAL RESEARCH REVIEWS 3: 341 (1983).","journal-title":"MEDICINAL RESEARCH REVIEWS"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1042\/bj2190763","volume":"219","year":"1984","unstructured":"CHARLIER, P, ACTIVE-SITE-DIRECTED INACTIVATORS OF THE ZN-2+-CONTAINING D-ALANYL-D-ALANINE-CLEAVING CARBOXYPEPTIDASE OF STREPTOMYCES-ALBUS-G, BIOCHEMICAL JOURNAL 219: 763 (1984).","journal-title":"BIOCHEMICAL JOURNAL"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0022-2836(83)80358-1","volume":"171","year":"1983","unstructured":"CHARLIER, P, CRYSTALLOGRAPHIC DATA FOR THE BETA-LACTAMASE FROM ENTEROBACTER-CLOACAE P99, JOURNAL OF MOLECULAR BIOLOGY 171: 237 (1983).","journal-title":"JOURNAL OF MOLECULAR BIOLOGY"},{"key":"e_1_2_1_7_1","first-page":"374","volume":"289","year":"1980","unstructured":"DELUCIA, M. L., PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 289: 374 (1980).","journal-title":"PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0022-2836(85)90333-X","volume":"181","year":"1985","unstructured":"DIDEBERG, O, CRYSTALLIZATION AND PRELIMINARY-X-RAY DATA FOR THE EXOCELLULAR BETA-LACTAMASE OF BACILLUS-LICHENIFORMIS 749\/C, JOURNAL OF MOLECULAR BIOLOGY 181: 145 (1985).","journal-title":"JOURNAL OF MOLECULAR BIOLOGY"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1038\/299469a0","volume":"299","year":"1982","unstructured":"DIDEBERG, O, STRUCTURE OF A ZN-2+-CONTAINING D-ALANYL-D-ALANINE-CLEAVING CARBOXYPEPTIDASE AT 2.5-A RESOLUTION, NATURE 299: 469 (1982).","journal-title":"NATURE"},{"key":"e_1_2_1_10_1","unstructured":"DUEZ C unpublished data."},{"key":"e_1_2_1_11_1","first-page":"299","volume":"11","year":"1985","unstructured":"FRERE, J. M., CRC CRITICAL REVIEWS IN MICROBIOLOGY 11: 299 (1985).","journal-title":"CRC CRITICAL REVIEWS IN MICROBIOLOGY"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0014-5793(76)80770-3","volume":"70","year":"1976","unstructured":"FRERE, J.M., OCCURRENCE OF A SERINE RESIDUE IN PENICILLIN-BINDING SITE OF EXOCELLULAR \"DD-CARBOXY-PEPTIDASE-TRANSPEPTIDASE FROM STREPTOMYCES R61, FEBS LETTERS 70: 257 (1976).","journal-title":"FEBS LETTERS"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1128\/br.32.4_pt_2.425-464.1968","volume":"32","year":"1968","unstructured":"GHUYSEN, J.M., USE OF BACTERIOLYTIC ENZYMES IN DETERMINATION OF WALL STRUCTURE AND THEIR ROLE IN CELL METABOLISM, BACTERIOLOGICAL REVIEWS 32: 425 (1968).","journal-title":"BACTERIOLOGICAL REVIEWS"},{"key":"e_1_2_1_14_1","first-page":"4897","volume":"78","year":"1981","unstructured":"Jaurin, B., Proceedings of the National Academy of Sciences of the United States of America 78: 4897 (1981).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_1_15_1","first-page":"1999","volume":"82","year":"1985","unstructured":"KECK, W, SEQUENCES OF THE ACTIVE-SITE PEPTIDES OF 3 OF THE HIGH-MR PENICILLIN-BINDING PROTEINS OF ESCHERICHIA-COLI K-12, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 1999 (1985).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"6449","DOI":"10.1016\/S0021-9258(18)88993-1","volume":"260","year":"1985","unstructured":"KELLY, J.A., 2.8-A STRUCTURE OF PENICILLIN-SENSITIVE D-ALANYL CARBOXYPEPTIDASE-TRANSPEPTIDASE FROM STREPTOMYCES-R61 AND COMPLEXES WITH BETA-LACTAMS, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 6449 (1985).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1126\/science.7123246","volume":"218","year":"1982","unstructured":"KELLY, J.A., PENICILLIN TARGET ENZYME AND THE ANTIBIOTIC BINDING-SITE, SCIENCE 218: 479 (1982).","journal-title":"SCIENCE"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1021\/ar00112a001","volume":"18","year":"1985","unstructured":"KNOWLES, J. R., ACCOUNTS OF CHEMICAL RESEARCH 18: 97 (1985).","journal-title":"ACCOUNTS OF CHEMICAL RESEARCH"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/0022-2836(76)90187-X","volume":"104","year":"1976","unstructured":"KNOX, J.R., 5.5 A CRYSTALLOGRAPHIC STRUCTURE OF PENICILLIN BETA-LACTAMASE AND RADIUS OF GYRATION IN SOLUTION, JOURNAL OF MOLECULAR BIOLOGY 104: 865 (1976).","journal-title":"JOURNAL OF MOLECULAR BIOLOGY"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1042\/bj2250167","volume":"225","year":"1985","unstructured":"MOULT, J, BIOCHEMICAL JOURNAL 225: 167 (1985).","journal-title":"BIOCHEMICAL JOURNAL"},{"key":"e_1_2_1_21_1","first-page":"1247","volume":"129","year":"1983","unstructured":"SPRATT, B.G., PENICILLIN-BINDING PROTEINS AND THE FUTURE OF BETA-LACTAM ANTIBIOTICS - THE 7TH FLEMING LECTURE, JOURNAL OF GENERAL MICROBIOLOGY 129: 1247 (1983).","journal-title":"JOURNAL OF GENERAL MICROBIOLOGY"},{"key":"e_1_2_1_22_1","first-page":"1133","volume":"54","year":"1965","unstructured":"TIPPER, D.J., MECHANISM OF ACTION OF PENICILLINS - A PROPOSAL BASED ON THEIR STRUCTURAL SIMILARITY TO ACYL-D-ALANYL-D-ALANINE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 54: 1133 (1965).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_23_1","unstructured":"WILLIAMS T.V. THESIS U N CAROLINA (1982)."}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.3082007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T21:16:18Z","timestamp":1705180578000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.3082007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,3,21]]},"references-count":23,"journal-issue":{"issue":"4744","published-print":{"date-parts":[[1986,3,21]]}},"alternative-id":["10.1126\/science.3082007"],"URL":"https:\/\/doi.org\/10.1126\/science.3082007","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,3,21]]}}}