{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T23:28:36Z","timestamp":1778110116891,"version":"3.51.4"},"reference-count":15,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4768","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1986,9,5]]},"abstract":"<jats:p>\n            Acetyl-CoA:\u03b1-glucosaminide\n            <jats:italic>N<\/jats:italic>\n            -acetyltransferase is a lysosomal-membrane enzyme deficient in a genetic disorder, Sanfilippo disease type C. The enzyme catalyzes the transfer of an acetyl group from cytoplasmic acetyl-coenzyme A (acetyl-CoA) to terminal \u03b1-glucosamine residues of heparan sulfate within the organelle. Previous kinetic experiments indicated that the enzyme carries out a transmembrane acetylation via a ping-pong mechanism; the reaction can therefore be dissected into two half reactions\u2014acetylation of the enzyme, and transfer of the acetyl group to glucosamine. Cells derived from patients were found to differ in their ability to perform each half reaction. Five cell lines (derived from three families) were able to catalyze acetylation of the lysosomal membrane and to carry out acetyl-CoA\/CoA exchange, whereas a sixth cell line was devoid of this activity.\n          <\/jats:p>","DOI":"10.1126\/science.3090688","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:52Z","timestamp":1160080132000},"page":"1087-1089","source":"Crossref","is-referenced-by-count":32,"title":["Genetic Evidence for Transmembrane Acetylation by Lysosomes"],"prefix":"10.1126","volume":"233","author":[{"given":"Karen J.","family":"Bame","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry and the Mental Retardation Research Center, School of Medicine, University of California, Los Angeles, CA 90024."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard H.","family":"Rome","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry and the Mental Retardation Research Center, School of Medicine, University of California, Los Angeles, CA 90024."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"1293","volume":"260","year":"1985","unstructured":"BAME, K.J., ACETYL COENZYME A-ALPHA-GLUCOSAMINIDE N-ACETYLTRANSFERASE - 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