{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:06:12Z","timestamp":1791194772531,"version":"4.1.0"},"reference-count":27,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4778","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1986,11,14]]},"abstract":"<jats:p>\n            Interleukin-2 (IL-2) binds to both high- and low-affinity classes of IL-2 receptors on activated T lymphocytes. Only the high-affinity receptors are involved in receptor-mediated endocytosis and normally transduce the mitogenic signals of IL-2; however, the structural features distinguishing the high- and low-affinity receptors are unknown. When\n            <jats:sup>125<\/jats:sup>\n            I-labeled IL-2 was chemically cross-linked to activated human T lymphocytes, two major bands were identified. First, as predicted, a 68- to 72-kilodalton band, consisting of IL-2 (15.5 kilodaltons) cross-linked to the IL-2 receptor (55 kilodaltons), was observed. Second, an unpredicted 85- to 92-kilodalton moiety was detected. This band was not present when IL-2 was cross-linked to transfected C127 cells, which exclusively express low-affinity receptors. The data presented are most consistent with the existence of a 70- to 77-kilodalton glycoprotein subunit (p70) which, upon associating with the 55-kilodalton low-affinity receptor (p55), transforms it into a high-affinity site. It is proposed that p55 and p70 be referred to as the \u03b1 and \u03b2 subunits, respectively, of the high-affinity IL-2 receptor.\n          <\/jats:p>","DOI":"10.1126\/science.3095922","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:52Z","timestamp":1160080132000},"page":"859-863","source":"Crossref","is-referenced-by-count":424,"title":["Novel Interleukin-2 Receptor Subunit Detected by Cross-Linking Under High-Affinity Conditions"],"prefix":"10.1126","volume":"234","author":[{"given":"Michael","family":"Sharon","sequence":"first","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Klausner","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan R.","family":"Cullen","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Hoffmann-La Roche, Inc., Nutley, NJ 07110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Chizzonite","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Hoffmann-La Roche, Inc., Nutley, NJ 07110."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warren J.","family":"Leonard","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0092-8674(85)90321-6","volume":"40","year":"1985","unstructured":"BRENNER, M.B., CROSS-LINKING OF HUMAN T-CELL RECEPTOR PROTEINS - ASSOCIATION BETWEEN THE T-CELL IDIOTYPE BETA-SUBUNIT AND THE T3 GLYCOPROTEIN HEAVY SUBUNIT, CELL 40: 183 (1985).","journal-title":"CELL"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/S0021-9258(19)75212-0","volume":"252","year":"1977","unstructured":"CLEVELAND, D.W., PEPTIDE MAPPING BY LIMITED PROTEOLYSIS IN SODIUM DODECYL-SULFATE AND ANALYSIS BY GEL-ELECTROPHORESIS, JOURNAL OF BIOLOGICAL CHEMISTRY 252: 1102 (1977).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/312768a0","volume":"312","year":"1984","unstructured":"COSMAN, D, CLONING, SEQUENCE AND EXPRESSION OF HUMAN INTERLEUKIN-2 RECEPTOR, NATURE 312: 768 (1984).","journal-title":"NATURE"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1084\/jem.163.3.550","volume":"163","year":"1986","unstructured":"FUJII, M, HIGH-AFFINITY RECEPTOR-MEDIATED INTERNALIZATION AND DEGRADATION OF INTERLEUKIN-2 IN HUMAN T-CELLS, JOURNAL OF EXPERIMENTAL MEDICINE 163: 550 (1986).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1084\/jem.162.1.363","volume":"162","year":"1985","unstructured":"GREENE, W.C., STABLE EXPRESSION OF CDNA-ENCODING THE HUMAN INTERLEUKIN-2 RECEPTOR IN EUKARYOTIC CELLS, JOURNAL OF EXPERIMENTAL MEDICINE 162: 363 (1985).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/318467a0","volume":"318","year":"1985","unstructured":"HATAKEYAMA, M, RECONSTITUTION OF FUNCTIONAL RECEPTOR FOR HUMAN INTERLEUKIN-2 IN MOUSE CELLS, NATURE 318: 467 (1985).","journal-title":"NATURE"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/320075a0","volume":"320","year":"1986","unstructured":"KONDO, S, EXPRESSION OF FUNCTIONAL HUMAN INTERLEUKIN-2 RECEPTOR IN MOUSE T-CELLS BY CDNA TRANSFECTION, NATURE 320: 75 (1986).","journal-title":"NATURE"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1016\/S0021-9258(18)89674-0","volume":"260","year":"1985","unstructured":"LEONARD, W.J., THE HUMAN RECEPTOR FOR T-CELL GROWTH-FACTOR - EVIDENCE FOR VARIABLE POST-TRANSLATIONAL PROCESSING, PHOSPHORYLATION, SULFATION, AND THE ABILITY OF PRECURSOR FORMS OF THE RECEPTOR TO BIND T-CELL GROWTH-FACTOR, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 1872 (1985).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/300267a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/311626a0"},{"key":"e_1_2_1_11_1","first-page":"6957","volume":"80","year":"1983","unstructured":"Leonard, W. 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