{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:27:07Z","timestamp":1787016427057,"version":"build-2736575974"},"reference-count":22,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4784","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1987,1,2]]},"abstract":"<jats:p>\n                    Two major protective antigens of\n                    <jats:italic>Rickettsia rickettsii<\/jats:italic>\n                    have been previously described. In this study, we cloned the gene encoding one of these antigens into\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and tested the effectiveness of the recombinant-made product as a vaccine for Rocky Mountain spotted fever. A clone bank of R strain\n                    <jats:italic>R. rickettsii<\/jats:italic>\n                    DNA was made in\n                    <jats:italic>E. coli<\/jats:italic>\n                    K-12 by using the plasmid vector pBR322. Transformants were screened for their ability to make rickettsial antigens by reactivity with rabbit antibodies to\n                    <jats:italic>R. rickettsii<\/jats:italic>\n                    . One of the transformants, EM24(pGAM21), made a product reactive with two monoclonal antibodies that recognize a 155-kilodalton protein of\n                    <jats:italic>R. rickettsii<\/jats:italic>\n                    . One of the monoclonal antibodies was a member of a class of antibodies that react to heat-sensitive epitopes and protect mice injected with a potentially lethal dose of viable\n                    <jats:italic>R. rickettsii<\/jats:italic>\n                    . The cloned product contained this protective heat-sensitive epitope. In order to obtain enhanced expression, the gene was subcloned downstream of the lactose promoter on the plasmid vector pUC8. A sonic lysate of\n                    <jats:italic>E. coli<\/jats:italic>\n                    harboring the pUC8 subclone was used successfully as a vaccine to protect mice injected with a lethal dose of the viable\n                    <jats:italic>R. rickettsii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1126\/science.3099387","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T16:28:58Z","timestamp":1160065738000},"page":"83-85","source":"Crossref","is-referenced-by-count":64,"title":["Cloned Gene of\n                    <i>Rickettsia rickettsii<\/i>\n                    Surface Antigen: Candidate Vaccine for Rocky Mountain Spotted Fever"],"prefix":"10.1126","volume":"235","author":[{"given":"Gregory A.","family":"McDonald","sequence":"first","affiliation":[{"name":"Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Anacker","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kareen","family":"Garjian","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Structure and Function, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"188","volume":"33","year":"1983","unstructured":"MORBIDITY AND MORTALITY WEEKLY REPORT 33: 188 (1983).","journal-title":"MORBIDITY AND MORTALITY WEEKLY REPORT"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1128\/iai.40.1.292-298.1983","volume":"40","year":"1983","unstructured":"ANACKER, R.L., BIOLOGICAL PROPERTIES OF RABBIT ANTIBODIES TO A SURFACE-ANTIGEN OF RICKETTSIA-RICKETTSII, INFECTION AND IMMUNITY 40: 292 (1983).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1128\/iai.51.2.653-660.1986","volume":"51","year":"1986","unstructured":"ANACKER, R.L., ANTIGENIC HETEROGENEITY IN HIGH-VIRULENCE AND LOW-VIRULENCE STRAINS OF RICKETTSIA-RICKETTSII REVEALED BY MONOCLONAL-ANTIBODIES, INFECTION AND IMMUNITY 51: 653 (1986).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1128\/iai.11.6.1203-1209.1975","volume":"11","year":"1975","unstructured":"ANACKER, R.L., IMMUNOLOGICAL PROPERTIES OF RICKETTSIA-RICKETTSII PURIFIED BY ZONAL CENTRIFUGATION, INFECTION AND IMMUNITY 11: 1203 (1975).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1093\/infdis\/151.6.1052","volume":"151","year":"1985","unstructured":"ANACKER, R.L., CHARACTERIZATION OF MONOCLONAL-ANTIBODIES PROTECTING MICE AGAINST RICKETTSIA-RICKETTSII, JOURNAL OF INFECTIOUS DISEASES 151: 1052 (1985).","journal-title":"JOURNAL OF INFECTIOUS DISEASES"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/0022-1759(82)90089-8","volume":"55","year":"1982","unstructured":"BATTEIGER, B, THE USE OF TWEEN-20 AS A BLOCKING-AGENT IN THE IMMUNOLOGICAL DETECTION OF PROTEINS TRANSFERRED TO NITROCELLULOSE MEMBRANES, JOURNAL OF IMMUNOLOGICAL METHODS 55: 297 (1982).","journal-title":"JOURNAL OF IMMUNOLOGICAL METHODS"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.4049\/jimmunol.87.6.737","volume":"87","year":"1961","unstructured":"BELL, E.J., SPOTTED FEVER VACCINE - POTENCY ASSAY BY DIRECT CHALLENGE OF VACCINATED MICE WITH TOXIN OF RICKETTSIA RICKETTSII, JOURNAL OF IMMUNOLOGY 87: 737 (1961).","journal-title":"JOURNAL OF IMMUNOLOGY"},{"key":"e_1_2_1_8_1","first-page":"103","volume":"34","year":"1977","unstructured":"BURGDORFER, W, ACTA TROP 34: 103 (1977).","journal-title":"ACTA TROP"},{"key":"e_1_2_1_9_1","unstructured":"Dasch G. A. paper presented at the Fifth National Confcrence of the American Society for Rickettsiology and Rickettsial Diseases Laguna Beach CA 1 to 3 March (1985)."},{"key":"e_1_2_1_10_1","unstructured":"Davis R. W. A Manual for Genetic Engineering: Advanced Bacterial Genetics (1980)."},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0378-1119(84)90135-5","volume":"27","year":"1984","unstructured":"DEBRUIJN, F.J., THE USE OF TRANSPOSON TN5 MUTAGENESIS IN THE RAPID GENERATION OF CORRELATED PHYSICAL AND GENETIC MAPS OF DNA SEGMENTS CLONED INTO MULTICOPY PLASMIDS - A REVIEW, GENE 27: 131 (1984).","journal-title":"GENE"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80031-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80284-8"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1093\/infdis\/150.4.480","volume":"150","year":"1984","unstructured":"HELMICK, C.G., ROCKY-MOUNTAIN SPOTTED-FEVER - CLINICAL, LABORATORY, AND EPIDEMIOLOGICAL FEATURES OF 262 CASES, JOURNAL OF INFECTIOUS DISEASES 150: 480 (1984).","journal-title":"JOURNAL OF INFECTIOUS DISEASES"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3969554"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_17_1","unstructured":"MCDONALD G.A. unpublished data."},{"key":"e_1_2_1_18_1","unstructured":"Silhavy T. 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Experiments with Gene Fusions (1984)."},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/iai.44.3.545-553.1984","volume":"44","year":"1984","unstructured":"SILVERMAN, D.J., RICKETTSIA-RICKETTSII-INDUCED CELLULAR INJURY OF HUMAN VASCULAR ENDOTHELIUM INVITRO, INFECTION AND IMMUNITY 44: 545 (1984).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_20_1","first-page":"311","volume":"40","year":"1983","unstructured":"TRINGALI, G.R., IMMUNITY AND RICKETTSIAL INFECTION - A REVIEW, ACTA TROPICA 40: 311 (1983).","journal-title":"ACTA TROPICA"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/0378-1119(82)90015-4","volume":"19","year":"1982","unstructured":"VIEIRA, J, THE PUC PLASMIDS, AN M13MP7-DERIVED SYSTEM FOR INSERTION MUTAGENESIS AND SEQUENCING WITH SYNTHETIC UNIVERSAL PRIMERS, GENE 19: 259 (1982).","journal-title":"GENE"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1093\/infdis\/150.4.465","volume":"150","year":"1984","unstructured":"WOODWARD, T.E., ROCKY-MOUNTAIN SPOTTED-FEVER - EPIDEMIOLOGICAL AND EARLY CLINICAL SIGNS ARE KEYS TO TREATMENT AND REDUCED MORTALITY, JOURNAL OF INFECTIOUS DISEASES 150: 465 (1984).","journal-title":"JOURNAL OF INFECTIOUS DISEASES"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.3099387","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T16:12:08Z","timestamp":1705162328000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.3099387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,1,2]]},"references-count":22,"journal-issue":{"issue":"4784","published-print":{"date-parts":[[1987,1,2]]}},"alternative-id":["10.1126\/science.3099387"],"URL":"https:\/\/doi.org\/10.1126\/science.3099387","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,1,2]]}}}