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Fetuses received continuous intracranial infusions of tetrodotoxin from osmotic minipumps implanted in utero on E42. After a 2-week infusion, intraocular injections of anterograde tracers revealed that tetrodotoxin prevented segregation. The contralateral projection filled the lateral geniculate nucleus uniformly, and the ipsilateral projection expanded to occupy most of what would normally be contralaterally innervated layer A. Thus, in the fetus, long before the onset of vision, spontaneous action potential activity is likely to be present in the visual system and to contribute to the segregation of the retinogeniculate pathway.<\/jats:p>","DOI":"10.1126\/science.3175636","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T21:21:15Z","timestamp":1160083275000},"page":"87-89","source":"Crossref","is-referenced-by-count":428,"title":["Prenatal Tetrodotoxin Infusion Blocks Segregation of Retinogeniculate Afferents"],"prefix":"10.1126","volume":"242","author":[{"given":"Carla J.","family":"Shatz","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Stryker","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"2891","DOI":"10.1523\/JNEUROSCI.04-12-02891.1984","volume":"4","year":"1984","unstructured":"BOSS, V.C., ACTIVITY AND THE FORMATION OF OCULAR DOMINANCE PATCHES IN DUALLY INNERVATED TECTUM OF GOLDFISH, JOURNAL OF NEUROSCIENCE 4: 2891 (1984).","journal-title":"JOURNAL OF NEUROSCIENCE"},{"key":"e_1_2_1_2_1","first-page":"589","volume":"12","year":"1986","unstructured":"CAMPBELL, G, SOCIETY FOR NEUROSCIENCE. 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