{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:18:14Z","timestamp":1790403494237,"version":"4.1.0"},"reference-count":25,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4860","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1988,6,24]]},"abstract":"<jats:p>\n            Platelet-activating factor (PAF) is a naturally occurring phospholipid that serves as a critical mediator in diverse biological and pathophysiological processes. In this study of the interactions of PAF with neuronal cells, it was found that PAF increased the intracellular levels of free calcium ions in cells of the clones NG108-15 and PC12. The increase was dependent on extracellular calcium and was inhibited by the antagonistic PAF analog CV-3988 and by the calcium-influx blockers prenylamine and diltiazem. A functional consequence of this interaction was revealed by measuring a PAF-elicited, Ca\n            <jats:sup>2+<\/jats:sup>\n            -dependent secretion of adenosine triphosphate from PC12 cells. Exposure of NG108-15 cells for 3 to 4 days to low concentrations of PAF induced neuronal differentiation; higher concentrations were neurotoxic. Thus, by influencing Ca\n            <jats:sup>2+<\/jats:sup>\n            fluxes, PAF may play a physiological role in neuronal development and a pathophysiological role in the degeneration that occurs when neurons are exposed to circulatory factors as a result of trauma, stroke, or spinal cord injury.\n          <\/jats:p>","DOI":"10.1126\/science.3381103","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T21:21:15Z","timestamp":1160083275000},"page":"1792-1794","source":"Crossref","is-referenced-by-count":197,"title":["Neuroregulatory and Neuropathological Actions of the Ether-Phospholipid Platelet-Activating Factor"],"prefix":"10.1126","volume":"240","author":[{"given":"Elizabeth","family":"Kornecki","sequence":"first","affiliation":[{"name":"Neuroscience Research Unit, Departments of Psychiatry and Biochemistry, University of Vermont College of Medicine, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yigal H.","family":"Ehrlich","sequence":"additional","affiliation":[{"name":"Neuroscience Research Unit, Departments of Psychiatry and Biochemistry, University of Vermont College of Medicine, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1084\/jem.136.6.1356","volume":"136","year":"1972","unstructured":"BENVENISTE, J, LEUKOCYTE-DEPENDENT HISTAMINE-RELEASE FROM RABBIT PLATELETS - ROLE OF IGE, BASOPHILS, AND A PLATELET-ACTIVATING FACTOR, JOURNAL OF EXPERIMENTAL MEDICINE 136: 1356 (1972).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_2_1","first-page":"1622","volume":"13","year":"1987","unstructured":"BERNARDINI, R, ABSTRACTS. SOCIETY FOR NEUROSCIENCE 13: 1622 (1987).","journal-title":"ABSTRACTS. SOCIETY FOR NEUROSCIENCE"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0021-9258(19)70115-X","volume":"256","year":"1981","unstructured":"BLANK, M.L., A SPECIFIC ACETYLHYDROLASE FOR 1-ALKYL-2-ACETYL-SN-GLYCERO-3-PHOSPHOCHOLINE (A HYPOTENSIVE AND PLATELET-ACTIVATING LIPID), JOURNAL OF BIOLOGICAL CHEMISTRY 256: 175 (1981).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0079-6107(82)90011-6","volume":"40","year":"1982","unstructured":"BLINKS, J.R., MEASUREMENT OF CA-2+ CONCENTRATIONS IN LIVING CELLS, PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY 40: 1 (1982).","journal-title":"PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"16502","DOI":"10.1016\/S0021-9258(18)66594-9","volume":"261","year":"1986","unstructured":"BUSSOLINO, F, PRODUCTION OF PLATELET-ACTIVATING-FACTOR BY CHICK RETINA, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 16502 (1986).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1471-4159.1988.tb13263.x","volume":"50","year":"1988","unstructured":"EHRLICH, Y.H., MODULATION OF NEURONAL SIGNAL TRANSDUCTION SYSTEMS BY EXTRACELLULAR ATP, JOURNAL OF NEUROCHEMISTRY 50: 295 (1988).","journal-title":"JOURNAL OF NEUROCHEMISTRY"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/320067a0","volume":"320","year":"1986","unstructured":"EHRLICH, Y.H., ECTOPROTEIN KINASE-ACTIVITY ON THE EXTERNAL SURFACE OF NEURAL CELLS, NATURE 320: 67 (1986).","journal-title":"NATURE"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0006-8993(77)90005-1","volume":"129","year":"1977","unstructured":"GREENE, L.A., RELEASE, STORAGE AND UPTAKE OF CATECHOLAMINES BY A CLONAL CELL LINE OF NERVE GROWTH-FACTOR (NGF) RESPONSIVE PHEOCHROMOCYTOMA CELLS, BRAIN RESEARCH 129: 247 (1977).","journal-title":"BRAIN RESEARCH"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1042\/bj2180819","volume":"218","year":"1984","unstructured":"HALLAM, T.J., STIMULUS-RESPONSE COUPLING IN HUMAN-PLATELETS - CHANGES EVOKED BY PLATELET-ACTIVATING FACTOR IN CYTOPLASMIC FREE CALCIUM MONITORED WITH THE FLUORESCENT CALCIUM INDICATOR QUIN2, BIOCHEMICAL JOURNAL 218: 819 (1984).","journal-title":"BIOCHEMICAL JOURNAL"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/0076-6879(85)09096-6","volume":"109","year":"1985","unstructured":"HAMPRECHT, B, CULTURE AND CHARACTERISTICS OF HORMONE-RESPONSIVE NEURO-BLASTOMA X GLIOMA HYBRID-CELLS, METHODS IN ENZYMOLOGY 109: 316 (1985).","journal-title":"METHODS IN ENZYMOLOGY"},{"key":"e_1_2_1_11_1","first-page":"3","year":"1987","unstructured":"Henson, P. 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