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Shared amino acid sequences from a 100-kilodalton plasma glycoprotein and from vW AgII are identical to amino acid sequences predicted from a complementary DNA clone encoding the 5\u2032 end of vWF. In addition, these proteins have identical molecular weights and immunologic cross reactivities. Monoclonal antibodies prepared against both proteins recognize epitopes on the pro-vWF subunit and on a 100-kilodalton protein that are not present on the mature vWF subunit in endothelial cell lysates. In contrast, polyclonal antibodies against vWF recognize both pro-vWF and vWF subunits. Thus, the 100-kilodalton plasma glycoprotein and vW AgII are identical proteins and represent an extremely large propolypeptide that is first cleaved from pro-vWF during intracellular processing and then released into plasma.<\/jats:p>","DOI":"10.1126\/science.3486471","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:52Z","timestamp":1160080132000},"page":"995-998","source":"Crossref","is-referenced-by-count":89,"title":["Propolypeptide of von Willebrand Factor Circulates in Blood and Is Identical to von Willebrand Antigen II"],"prefix":"10.1126","volume":"232","author":[{"given":"Philip J.","family":"Fay","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Rochester Medical Center, Rochester , NY 14642."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohko","family":"Kawai","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics, Medicine, and Pathology, Medical College of Wisconsin, Milwaukee, WI 53233, and the Blood Center of Southeastern Wisconsin, Milwaukee, WI 53233."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denisa D.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Rochester Medical Center, Rochester , NY 14642."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Ginsburg","sequence":"additional","affiliation":[{"name":"Hematology Divisions, Brigham and Women's Hospital, and Departments of Medicine and Pediatrics, Harvard Medical Sool, Boston, MA 02115."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bonthron","sequence":"additional","affiliation":[{"name":"Hematology Division, Children's Hospital, and Departments of Medicine and Pediatrics, Harvard Medical School, Boston, MA 02115."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betsy M.","family":"Ohlsson-Wilhelm","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Rochester Medical Center, Rochester, NY 14642."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen I.","family":"Chavin","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Rochester Medical Center, Rochester , NY 14642."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George N.","family":"Abraham","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Rochester Medical Center, Rochester , NY 14642."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert I.","family":"Handin","sequence":"additional","affiliation":[{"name":"Hematology Divisions, Brigham and Women's Hospital and Children's Hospital, and Departments of Medicine and Pediatrics, Harvard Medical School, Boston, MA 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