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Furthermore, CaM-dependent enzyme systems were inhibited equally by active and inactive cyclosporines, but only at concentrations 100 times those necessary to block lymphocyte activation. Thus the exquisite immunosuppressive stereospecificity displayed by cyclosporine isomers is not reflected in the binding to and inhibition of CaM, suggesting that inhibition of CaM-dependent processes is not sufficient to explain the immunosuppressive activity of CsA.<\/jats:p>","DOI":"10.1126\/science.3749892","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:28:52Z","timestamp":1160080132000},"page":"68-71","source":"Crossref","is-referenced-by-count":61,"title":["Does the Binding of Cyclosporine to Calmodulin Result in Immunosuppression?"],"prefix":"10.1126","volume":"234","author":[{"given":"Stephen J.","family":"LeGrue","sequence":"first","affiliation":[{"name":"Department of Immunology, The University of Texas M. D. Anderson Hospital and Tumor Institute (Box 178), Houston, TX 77030."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodney","family":"Turner","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Cell Biology, The University of Texas Medical School, Houston, TX 77025."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman","family":"Weisbrodt","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Cell Biology, The University of Texas Medical School, Houston, TX 77025."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Dedman","sequence":"additional","affiliation":[{"name":"Departments of Physiology and Cell Biology, The University of Texas Medical School, Houston, TX 77025."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"C255","DOI":"10.1152\/ajpcell.1983.245.3.C255","volume":"245","year":"1983","unstructured":"AKSOY, M.O., CA-2+, CAMP, AND CHANGES IN MYOSIN PHOSPHORYLATION DURING CONTRACTION OF SMOOTH-MUSCLE, AMERICAN JOURNAL OF PHYSIOLOGY 245: C255 (1983).","journal-title":"AMERICAN JOURNAL OF PHYSIOLOGY"},{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/312315a0"},{"key":"e_1_2_1_3_1","first-page":"2219","volume":"15","year":"1983","unstructured":"BOREL, J.F., CYCLOSPORINE - HISTORICAL PERSPECTIVES, TRANSPLANTATION PROCEEDINGS 15: 2219 (1983).","journal-title":"TRANSPLANTATION PROCEEDINGS"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1126\/science.3885394","volume":"228","year":"1985","unstructured":"COLOMBANI, P.M., CYCLOSPORIN-A BINDING TO CALMODULIN - A POSSIBLE SITE OF ACTION ON LYMPHOCYTES-T, SCIENCE 228: 337 (1985).","journal-title":"SCIENCE"},{"key":"e_1_2_1_5_1","unstructured":"DEDMAN J.R. unpublished data."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6334364"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"627","DOI":"10.4049\/jimmunol.116.3.627","volume":"116","year":"1976","unstructured":"FURGUSON, R, J IMMUNOL 116: 627 (1976).","journal-title":"J IMMUNOL"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1084\/jem.160.6.1792","volume":"160","year":"1984","unstructured":"GRANELLIPIPERNO, A, STIMULATION OF LYMPHOKINE RELEASE FROM T-LYMPHOBLASTS - REQUIREMENT FOR MESSENGER-RNA SYNTHESIS AND INHIBITION BY CYCLOSPORIN-A, JOURNAL OF EXPERIMENTAL MEDICINE 160: 1792 (1984).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6238408"},{"key":"e_1_2_1_10_1","first-page":"125","volume":"97","year":"1985","unstructured":"KAHAN, B.D., CLINICAL AND EXPERIMENTAL STUDIES WITH CYCLOSPORINE IN RENAL-TRANSPLANTATION, SURGERY 97: 125 (1985).","journal-title":"SURGERY"},{"key":"e_1_2_1_11_1","first-page":"1193","volume":"83","year":"1986","unstructured":"KINCAID, R.L., DIRECT COMPARISON OF CA-2+ REQUIREMENTS FOR CALMODULIN INTERACTION WITH AND ACTIVATION OF PROTEIN PHOSPHATASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 1193 (1986).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_12_1","first-page":"5214","volume":"81","year":"1984","unstructured":"Kr\u00f6nke, M., Proceedings of the National Academy of Sciences of the United States of America 81: 5214 (1984).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"712","DOI":"10.4049\/jimmunol.131.2.712","volume":"131","year":"1983","unstructured":"LEGRUE, S.J., BINDING OF CYCLOSPORINE BY HUMAN-LYMPHOCYTES AND PHOSPHOLIPID-VESICLES, JOURNAL OF IMMUNOLOGY 131: 712 (1983).","journal-title":"JOURNAL OF IMMUNOLOGY"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1097\/00007890-198612000-00019","volume":"42","year":"1986","unstructured":"LeGrue, S. 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