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The cloned MCF-\n            <jats:sub>ras<\/jats:sub>\n            transfectants, which expressed the v-\n            <jats:italic>ras<\/jats:italic>\n            <jats:sup>H<\/jats:sup>\n            messenger RNA and v-\n            <jats:italic>ras<\/jats:italic>\n            <jats:sup>H<\/jats:sup>\n            p21 protein (21,000 daltons), were characterized. In contrast to the parental cell line, MCF-\n            <jats:sub>ras<\/jats:sub>\n            cells no longer responded to exogenous estrogen in culture and their growth was minimally inhibited by exogenous antiestrogens. When tested in the nude mouse, the MCF-\n            <jats:sub>ras<\/jats:sub>\n            cells were fully tumorigenic in the absence of estrogen supplementation. Thus, cells acquiring an activated onc gene can bypass the hormonal regulatory signals that trigger the neoplastic growth of a human breast cancer cell line.\n          <\/jats:p>","DOI":"10.1126\/science.4039465","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T19:42:50Z","timestamp":1160077370000},"page":"725-728","source":"Crossref","is-referenced-by-count":137,"title":["Transfection of v-\n            <i>ras<\/i>\n            <sup>H<\/sup>\n            DNA into MCF-7 Human Breast Cancer Cells Bypasses Dependence on Estrogen for Tumorigenicity"],"prefix":"10.1126","volume":"228","author":[{"given":"Attan","family":"Kasid","sequence":"first","affiliation":[{"name":"Medical Breast Cancer Section, Medicine Branch, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc E.","family":"Lippman","sequence":"additional","affiliation":[{"name":"Medical Breast Cancer Section, Medicine Branch, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex G.","family":"Papageorge","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas R.","family":"Lowy","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward P.","family":"Gelmann","sequence":"additional","affiliation":[{"name":"Medical Breast Cancer Section, Medicine Branch, National Cancer Institute, Bethesda, Maryland 20205"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1016\/S0006-291X(80)80016-7","volume":"97","year":"1980","unstructured":"ADAMS, D.J., ESTROGEN REGULATION OF SPECIFIC MESSENGER-RNA IN HUMAN-BREAST CANCER-CELLS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 97: 1354 (1980).","journal-title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/308069a0","volume":"308","year":"1984","unstructured":"ALBINO, A.P., TRANSFORMING RAS GENES FROM HUMAN-MELANOMA - 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