{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:31:47Z","timestamp":1762075907209},"reference-count":3,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"4369","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1978,11,17]]},"abstract":"<jats:p>\n            A new approach to the culture of African trypanosomes led to the growth of the infective forms of the causative agent of human African trypanosomiasis. Infective cultures of\n            <jats:italic>Trypanosoma rhodesiense<\/jats:italic>\n            were initiated and maintained in vitro on Chinese hamster lung cells. By changing daily one-third of the Hepes-buffered RPMI 1640 medium containing 20 percent fetal bovine serum, the trypanosome numbers increased to 3 \u00d7 10\n            <jats:sup>6<\/jats:sup>\n            to 5 \u00d7 10\n            <jats:sup>6<\/jats:sup>\n            cells per milliliter. After 80 days in vitro at 37\u00b0C, the cultured trypomastigotes are infective for mice and rats and morphologically similar to bloodstream trypomastigotes in having a subterminal kinetoplast and a surface coat. In addition, they possess L-\u03b1-glycerophosphate oxidase, the predominant steady-state terminal oxidase of bloodstream trypomastigotes.\n          <\/jats:p>","DOI":"10.1126\/science.715441","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T16:35:25Z","timestamp":1160066125000},"page":"763-765","source":"Crossref","is-referenced-by-count":25,"title":["Growth of Infective Forms of\n            <i>Trypanosoma rhodesiense<\/i>\n            in Vitro, the Causative Agent of African Trypanosomiasis"],"prefix":"10.1126","volume":"202","author":[{"given":"George C.","family":"Hill","sequence":"first","affiliation":[{"name":"Department of Pathology, Graduate Program in Cell and Molecular Biology, Colorado State University, Fort Collins 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan Peterlin","family":"Shimer","sequence":"additional","affiliation":[{"name":"Department of Pathology, Graduate Program in Cell and Molecular Biology, Colorado State University, Fort Collins 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byron","family":"Caughey","sequence":"additional","affiliation":[{"name":"Department of Pathology, Graduate Program in Cell and Molecular Biology, Colorado State University, Fort Collins 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Scott","family":"Sauer","sequence":"additional","affiliation":[{"name":"Department of Pathology, Graduate Program in Cell and Molecular Biology, Colorado State University, Fort Collins 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","first-page":"31","year":"1976","unstructured":"HILL, G.C., BIOCH PARASITE HOST: 31 (1976).","journal-title":"BIOCH PARASITE HOST"},{"key":"e_1_2_1_2_1","first-page":"405","volume":"55","year":"1977","unstructured":"HIRUMI, H, CULTIVATION OF BLOOD-STREAM TRYPANOSOMA-BRUCEI, BULLETIN OF THE WORLD HEALTH ORGANIZATION 55: 405 (1977).","journal-title":"BULLETIN OF THE WORLD HEALTH ORGANIZATION"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.558652"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.715441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,14]],"date-time":"2024-01-14T22:56:01Z","timestamp":1705272961000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.715441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,11,17]]},"references-count":3,"journal-issue":{"issue":"4369","published-print":{"date-parts":[[1978,11,17]]}},"alternative-id":["10.1126\/science.715441"],"URL":"https:\/\/doi.org\/10.1126\/science.715441","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,11,17]]}}}