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When applied to a murine model of typhoid fever caused by\n                    <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    , mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the\n                    <jats:italic>inv\/spa<\/jats:italic>\n                    family of\n                    <jats:italic>S. typhimurium<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1126\/science.7618105","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T14:19:42Z","timestamp":1161958782000},"page":"400-403","source":"Crossref","is-referenced-by-count":1036,"title":["Simultaneous Identification of Bacterial Virulence Genes by Negative Selection"],"prefix":"10.1126","volume":"269","author":[{"given":"Michael","family":"Hensel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline E.","family":"Shea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Gleeson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Dalton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Holden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1128\/iai.62.5.1623-1630.1994","volume":"62","year":"1994","unstructured":"BAUMLER, A.J., SALMONELLA-TYPHIMURIUM LOCI INVOLVED IN SURVIVAL WITHIN MACROPHAGES, INFECTION AND IMMUNITY 62: 1623 (1994).","journal-title":"INFECTION AND IMMUNITY"},{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1111\/j.1365-2958.1993.tb01184.x","volume":"7","year":"1993","unstructured":"BUCHMEIER, N.A., RECOMBINATION-DEFICIENT MUTANTS OF SALMONELLA-TYPHIMURIUM ARE AVIRULENT AND SENSITIVE TO THE OXIDATIVE BURST OF MACROPHAGES, MOLECULAR MICROBIOLOGY 7: 933 (1993).","journal-title":"MOLECULAR MICROBIOLOGY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1084\/jem.139.5.1189","volume":"139","year":"1974","unstructured":"CARTER, P.B., ROUTE OF ENTERIC INFECTION IN NORMAL MICE, JOURNAL OF EXPERIMENTAL MEDICINE 139: 1189 (1974).","journal-title":"JOURNAL OF EXPERIMENTAL MEDICINE"},{"key":"e_1_2_1_4_1","unstructured":"Davis R. 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