{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:35:02Z","timestamp":1791326102441,"version":"4.3.1"},"reference-count":24,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5224","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1995,8,4]]},"abstract":"<jats:p>\n            The related mouse\n            <jats:italic>Engrailed<\/jats:italic>\n            genes\n            <jats:italic>En-1<\/jats:italic>\n            and\n            <jats:italic>En-2<\/jats:italic>\n            are expressed from the one- and approximately five-somite stages, respectively, in a similar presumptive mid-hindbrain domain. However, mutations in\n            <jats:italic>En-1<\/jats:italic>\n            and\n            <jats:italic>En-2<\/jats:italic>\n            produce different phenotypes.\n            <jats:italic>En-1<\/jats:italic>\n            mutant mice die at birth with a large mid-hindbrain deletion, whereas\n            <jats:italic>En-2<\/jats:italic>\n            mutants are viable, with cerebellar defects. To determine whether these contrasting phenotypes reflect differences in temporal expression or biochemical activity of the En proteins,\n            <jats:italic>En-1<\/jats:italic>\n            coding sequences were replaced with\n            <jats:italic>En-2<\/jats:italic>\n            sequences by gene targeting. This rescued all\n            <jats:italic>En-1<\/jats:italic>\n            mutant defects, demonstrating that the difference between\n            <jats:italic>En-1<\/jats:italic>\n            and\n            <jats:italic>En-2<\/jats:italic>\n            stems from their divergent expression patterns.\n          <\/jats:p>","DOI":"10.1126\/science.7624797","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T18:19:42Z","timestamp":1161973182000},"page":"679-682","source":"Crossref","is-referenced-by-count":357,"title":["Rescue of the\n            <i>En-1<\/i>\n            Mutant Phenotype by Replacement of\n            <i>En-1<\/i>\n            with\n            <i>En-2<\/i>"],"prefix":"10.1126","volume":"269","author":[{"given":"Mark","family":"Hanks","sequence":"first","affiliation":[{"name":"Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto M5G 1X5, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Wurst","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto M5G 1X5, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn","family":"Anson-Cartwright","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto M5G 1X5, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna B.","family":"Auerbach","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto M5G 1X5, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra L.","family":"Joyner","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mt. 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