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A strong constraint was placed on the Shaker channel pore-forming region by requiring its amino-terminal border to be 12 to 15 angstroms from the central axis. This method is directly applicable to sodium, calcium, and other ion channels where inhibitor or modulatory proteins bind with high affinity.<\/jats:p>","DOI":"10.1126\/science.7716527","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T14:19:42Z","timestamp":1161958782000},"page":"307-310","source":"Crossref","is-referenced-by-count":406,"title":["Revealing the Architecture of a K\n                    <sup>+<\/sup>\n                    Channel Pore Through Mutant Cycles with a Peptide Inhibitor"],"prefix":"10.1126","volume":"268","author":[{"given":"Patricia","family":"Hidalgo","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roderick","family":"MacKinnon","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 02115, 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