{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:47:03Z","timestamp":1770720423945,"version":"3.49.0"},"reference-count":29,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"5107","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[1993,4,23]]},"abstract":"<jats:p>\n            The CD45 protein is a transmembrane tyrosine phosphatase that is required for normal T cell receptor (TCR)-mediated signaling. A chimeric complementary DNA encoding the intracellular enzymatically active portion of murine CD45 preceded by a short amino-terminal sequence from p60\n            <jats:sup>\n              c-\n              <jats:italic>src<\/jats:italic>\n            <\/jats:sup>\n            was transfected into CD45- T cells. Expression of this chimeric protein corrected most of the TCR signaling abnormalities observed in the absence of CD45, including TCR-mediated enhancement of tyrosine kinase activity and Ca\n            <jats:sup>2+<\/jats:sup>\n            flux. Thus, the enzymatically active intracellular portion of CD45 is sufficient to allow TCR transmembrane signaling.\n          <\/jats:p>","DOI":"10.1126\/science.8475386","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T23:05:09Z","timestamp":1160089509000},"page":"541-544","source":"Crossref","is-referenced-by-count":93,"title":["Regulation of TCR Signaling by CD45 Lacking Transmembrane and Extracellular Domains"],"prefix":"10.1126","volume":"260","author":[{"given":"Sini\u0161a","family":"Volarevi\u0107","sequence":"first","affiliation":[{"name":"Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara B.","family":"Niklinska","sequence":"additional","affiliation":[{"name":"Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher M.","family":"Burns","sequence":"additional","affiliation":[{"name":"Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl H.","family":"June","sequence":"additional","affiliation":[{"name":"mmune Cell Biology Program, Naval Medical Research Institute, Bethesda, MD 20889."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan M.","family":"Weissman","sequence":"additional","affiliation":[{"name":"Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan D.","family":"Ashwell","sequence":"additional","affiliation":[{"name":"Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1126\/science.1323144","volume":"257","year":"1992","unstructured":"CENCIARELLI, C, ACTIVATION-INDUCED UBIQUITINATION OF THE T-CELL ANTIGEN RECEPTOR, SCIENCE 257: 795 (1992).","journal-title":"SCIENCE"},{"key":"e_1_2_1_2_1","first-page":"1834","volume":"4","year":"1984","unstructured":"CROSS, F.R., A SHORT SEQUENCE IN THE P60SRC N-TERMINUS IS REQUIRED FOR P60SRC MYRISTYLATION AND MEMBRANE ASSOCIATION AND FOR CELL-TRANSFORMATION, MOLECULAR AND CELLULAR BIOLOGY 4: 1834 (1984).","journal-title":"MOLECULAR AND CELLULAR BIOLOGY"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1002\/eji.1830201014","volume":"20","year":"1990","unstructured":"DIANZANI, U, MOLECULAR ASSOCIATIONS ON THE T-CELL SURFACE CORRELATE WITH IMMUNOLOGICAL MEMORY, EUROPEAN JOURNAL OF IMMUNOLOGY 20: 2249 (1990).","journal-title":"EUROPEAN JOURNAL OF IMMUNOLOGY"},{"key":"e_1_2_1_4_1","first-page":"4831","volume":"84","year":"1987","unstructured":"GUNNING, P, A HUMAN BETA-ACTIN EXPRESSION VECTOR SYSTEM DIRECTS HIGH-LEVEL ACCUMULATION OF ANTISENSE TRANSCRIPTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 4831 (1987).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"10836","DOI":"10.1016\/S0021-9258(18)81696-9","volume":"264","year":"1989","unstructured":"HSI, E.D., T-CELL ACTIVATION INDUCES RAPID TYROSINE PHOSPHORYLATION OF A LIMITED NUMBER OF CELLULAR SUBSTRATES, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 10836 (1989).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/0092-8674(89)90496-0","volume":"58","year":"1989","unstructured":"HUNTER, T, PROTEIN-TYROSINE PHOSPHATASES - 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