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The HPRT pattern of the hemolyzate from a Lesch-Nyhan patient was shifted to a more basic isoelectric\n            <jats:italic>p<\/jats:italic>\n            H, implying the mutation of a structural gene.\n          <\/jats:p>","DOI":"10.1126\/science.870972","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T16:03:07Z","timestamp":1160064187000},"page":"1119-1120","source":"Crossref","is-referenced-by-count":22,"title":["Hypoxanthine Phosphoribosyltransferase: Two-Dimensional Gels from Normal and Lesch-Nyhan Hemolyzates"],"prefix":"10.1126","volume":"196","author":[{"given":"Gurdev S.","family":"Ghangas","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Milman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland21205"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"crossref","first-page":"7398","DOI":"10.1016\/S0021-9258(19)45899-7","volume":"246","year":"1971","unstructured":"ARNOLD, W.J., HUMAN HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE - PURIFICATION AND SUBUNIT STRUCTURE, JOURNAL OF BIOLOGICAL CHEMISTRY 246: 7398 (1971).","journal-title":"JOURNAL OF BIOLOGICAL CHEMISTRY"},{"key":"e_1_2_1_2_1","first-page":"4147","volume":"72","year":"1975","unstructured":"GHANGAS, G.S., RADIOIMMUNE DETERMINATION OF HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE CROSSREACTING MATERIAL IN ERYTHROCYTES OF LESCH-NYHAN PATIENTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 72: 4147 (1975).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(64)90104-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1126\/science.171.3972.689","volume":"171","year":"1971","unstructured":"MCDONALD, J.A., LESCH-NYHAN SYNDROME - ALTERED KINETIC PROPERTIES OF MUTANT ENZYME, SCIENCE 171: 689 (1971).","journal-title":"SCIENCE"},{"key":"e_1_2_1_5_1","first-page":"4589","volume":"73","year":"1976","unstructured":"MILMAN, G, ANALYSIS OF HELA-CELL HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE MUTANTS AND REVERTANTS BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS - EVIDENCE FOR SILENT GENE ACTIVATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 4589 (1976).","journal-title":"PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_1_7_1","unstructured":"OLSEN A.S. 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