{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T01:47:26Z","timestamp":1792547246286,"version":"4.5.0"},"reference-count":40,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6388","funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI094973"],"award-info":[{"award-number":["AI094973"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI117017"],"award-info":[{"award-number":["AI117017"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["098051"],"award-info":[{"award-number":["098051"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2018,5,4]]},"abstract":"<jats:title>Saturating malaria mutagenesis<\/jats:title>\n                  <jats:p>\n                    Malaria is caused by eukaryotic\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    spp. parasites that classically infect red blood cells. These are difficult organisms to investigate genetically because of their AT-rich genomes. Zhang\n                    <jats:italic>et al.<\/jats:italic>\n                    have exploited this peculiarity by using\n                    <jats:italic>piggyBac<\/jats:italic>\n                    transposon insertion sites to achieve saturation-level mutagenesis for identifying and ranking essential genes and drug targets (see the Perspective by White and Rathod). Genes that are current candidates for drug targets were identified as essential, in contrast to many vaccine target genes. Notably, the proteasome degradation pathway was confirmed as a target for developing therapeutic interventions because of the several essential genes involved and the link to the mechanism of action of the current frontline drug, artemisinin.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Science<\/jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" related-article-type=\"in-this-issue\" xlink:href=\"10.1126\/science.aap7847\">eaap7847<\/jats:related-article>\n                    ; see also p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6388\" page=\"490\" related-article-type=\"in-this-issue\" vol=\"360\" xlink:href=\"10.1126\/science.aat5092\">490<\/jats:related-article>\n                  <\/jats:p>","DOI":"10.1126\/science.aap7847","type":"journal-article","created":{"date-parts":[[2018,5,3]],"date-time":"2018-05-03T14:13:28Z","timestamp":1525356808000},"source":"Crossref","is-referenced-by-count":1005,"title":["Uncovering the essential genes of the human malaria parasite\n                    <i>Plasmodium falciparum<\/i>\n                    by saturation mutagenesis"],"prefix":"10.1126","volume":"360","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5443-0992","authenticated-orcid":true,"given":"Min","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Global Health and Infectious Diseases, Department of Global Health, University of South Florida, 3720 Spectrum Boulevard, Suite 404, Tampa, FL 33612, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Global Health and Infectious Diseases, Department of Global Health, University of South Florida, 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