{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T15:36:43Z","timestamp":1791992203132,"version":"4.4.0"},"reference-count":27,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6431","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F32 DK101188"],"award-info":[{"award-number":["F32 DK101188"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["I-1544"],"award-info":[{"award-number":["I-1544"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001021","name":"Damon Runyon Cancer Research Foundation","doi-asserted-by":"publisher","award":["DRG-2249-16"],"award-info":[{"award-number":["DRG-2249-16"]}],"id":[{"id":"10.13039\/100001021","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2019,3,8]]},"abstract":"Organized for action<\/jats:title>\n \n It is becoming increasingly clear that biomolecular condensates, which are concentrations of macromolecules not surrounded by a membrane, are a key organizational structure in eukaryotic cells (see the Perspective by Martin and Mittag). Now, two papers show how such condensates function in actin assembly or in a Ras signaling pathway. In both cases, the condensates form at the plasma membrane and increase the activity of signaling proteins by increasing their membrane dwell times. Case\n et al.<\/jats:italic>\n show that the dwell time is dependent on cluster stoichiometry, so that stoichiometry of regulatory proteins can control actin assembly. Huang\n et al.<\/jats:italic>\n demonstrate that the longer dwell time allows kinetic proofreading in receptor-mediated activation of Ras.\n <\/jats:p>\n \n Science<\/jats:italic>\n , this issue p.\n 1093<\/jats:related-article>\n , p.\n 1098<\/jats:related-article>\n ; see also p.\n 1036<\/jats:related-article>\n <\/jats:p>","DOI":"10.1126\/science.aau6313","type":"journal-article","created":{"date-parts":[[2019,3,7]],"date-time":"2019-03-07T19:19:09Z","timestamp":1551986349000},"page":"1093-1097","source":"Crossref","is-referenced-by-count":555,"title":["Stoichiometry controls activity of phase-separated clusters of actin signaling proteins"],"prefix":"10.1126","volume":"363","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3166-1138","authenticated-orcid":true,"given":"Lindsay B.","family":"Case","sequence":"first","affiliation":[{"name":"The HHMI Summer Institute, Marine Biological Laboratory, Woods Hole, MA 02543, USA."},{"name":"Department of Biophysics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8287-7700","authenticated-orcid":true,"given":"Jonathon A.","family":"Ditlev","sequence":"additional","affiliation":[{"name":"The HHMI Summer Institute, Marine Biological Laboratory, Woods Hole, MA 02543, USA."},{"name":"Department of Biophysics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0775-7917","authenticated-orcid":true,"given":"Michael K.","family":"Rosen","sequence":"additional","affiliation":[{"name":"The HHMI Summer Institute, Marine Biological Laboratory, Woods Hole, MA 02543, USA."},{"name":"Department of Biophysics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf4382"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10879"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.04123"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad9964"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","unstructured":"S. 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Rosen BioRxiv 558965 [Preprint] (2019). https:\/\/doi.org\/10.1101\/558965","DOI":"10.1101\/558965"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.10.012"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100236108"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja029469f"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1602602113"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau5721"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12226"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717594115"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01008"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00603-0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1211164110"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1203397109"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2015.12.026"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201111113"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.29140"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2685"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","unstructured":"L. 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