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Available methods to find off-targets generally do not work in vivo or detect single-nucleotide changes. Three papers in this issue report new methods for monitoring gene-editing tools in vivo (see the Perspective by Kempton and Qi). Wienert\n                    <jats:italic>et al.<\/jats:italic>\n                    followed the recruitment of a DNA repair protein to DNA breaks induced by CRISPR-Cas9, enabling unbiased detection of off-target editing in cellular and animal models. Zuo\n                    <jats:italic>et al.<\/jats:italic>\n                    identified off-targets without the interference of natural genetic heterogeneity by injecting base editors into one blastomere of a two-cell mouse embryo and leaving the other genetically identical blastomere unedited. Jin\n                    <jats:italic>et al.<\/jats:italic>\n                    performed whole-genome sequencing on individual, genome-edited rice plants to identify unintended mutations. Cytosine, but not adenine, base editors induced numerous single-nucleotide variants in both mouse and rice.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Science<\/jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6437\" page=\"286\" related-article-type=\"in-this-issue\" vol=\"364\" xlink:href=\"10.1126\/science.aav9023\">286<\/jats:related-article>\n                    , p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6437\" page=\"289\" related-article-type=\"in-this-issue\" vol=\"364\" xlink:href=\"10.1126\/science.aav9973\">289<\/jats:related-article>\n                    , p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6437\" page=\"292\" related-article-type=\"in-this-issue\" vol=\"364\" xlink:href=\"10.1126\/science.aaw7166\">292<\/jats:related-article>\n                    ; see also p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6437\" page=\"234\" related-article-type=\"in-this-issue\" vol=\"364\" xlink:href=\"10.1126\/science.aax1827\">234<\/jats:related-article>\n                  <\/jats:p>","DOI":"10.1126\/science.aav9023","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T04:03:20Z","timestamp":1634789000000},"page":"286-289","source":"Crossref","is-referenced-by-count":404,"title":["Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq"],"prefix":"10.1126","volume":"364","author":[{"given":"Beeke","family":"Wienert","sequence":"first","affiliation":[{"name":"Innovative Genomics Institute, University of California Berkeley, Berkeley, CA 94704, USA."},{"name":"Department of Molecular 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USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2707-6035","authenticated-orcid":true,"given":"Charles D.","family":"Yeh","sequence":"additional","affiliation":[{"name":"Innovative Genomics Institute, University of California Berkeley, Berkeley, CA 94704, USA."},{"name":"Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinar","family":"Akcakaya","sequence":"additional","affiliation":[{"name":"Discovery Biology, AstraZeneca, 43150 Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1700-0085","authenticated-orcid":true,"given":"Michelle J.","family":"Porritt","sequence":"additional","affiliation":[{"name":"Discovery Biology, AstraZeneca, 43150 Gothenburg, 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