{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T10:05:50Z","timestamp":1792749950793,"version":"4.5.0"},"reference-count":33,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6510","license":[{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1453358"],"award-info":[{"award-number":["1453358"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-AI127521"],"award-info":[{"award-number":["R01-AI127521"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM120554"],"award-info":[{"award-number":["GM120554"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM124141"],"award-info":[{"award-number":["GM124141"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI122753"],"award-info":[{"award-number":["AI122753"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["F-1808"],"award-info":[{"award-number":["F-1808"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["F-1767"],"award-info":[{"award-number":["F-1767"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Science"],"published-print":{"date-parts":[[2020,9,18]]},"abstract":"<jats:title>Stabilizing the prefusion SARS-CoV-2 spike<\/jats:title>\n                  <jats:p>\n                    The development of therapeutic antibodies and vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is focused on the spike (S) protein that decorates the viral surface. A version of the spike ectodomain that includes two proline substitutions (S-2P) and stabilizes the prefusion conformation has been used to determine high-resolution structures. However, even S-2P is unstable and difficult to produce in mammalian cells. Hsieh\n                    <jats:italic>et al.<\/jats:italic>\n                    characterized many individual and combined structure-guided substitutions and identified a variant, named HexaPro, that retains the prefusion conformation but shows higher expression than S-2P and can also withstand heating and freezing. This version of the protein is likely to be useful in the development of vaccines and diagnostics.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Science<\/jats:italic>\n                    , this issue p.\n                    <jats:related-article xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" issue=\"6510\" page=\"1501\" related-article-type=\"in-this-issue\" vol=\"369\" xlink:href=\"10.1126\/science.abd0826\">1501<\/jats:related-article>\n                  <\/jats:p>","DOI":"10.1126\/science.abd0826","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T19:05:10Z","timestamp":1595531110000},"page":"1501-1505","source":"Crossref","is-referenced-by-count":1386,"title":["Structure-based design of prefusion-stabilized SARS-CoV-2 spikes"],"prefix":"10.1126","volume":"369","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3665-5717","authenticated-orcid":true,"given":"Ching-Lin","family":"Hsieh","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, TX 78712, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jory A.","family":"Goldsmith","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, TX 78712, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1130-2675","authenticated-orcid":true,"given":"Jeffrey M.","family":"Schaub","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, TX 78712, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea M.","family":"DiVenere","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, 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