{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T02:55:06Z","timestamp":1792378506196,"version":"4.4.0"},"reference-count":157,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6673","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Science"],"published-print":{"date-parts":[[2023,11,24]]},"abstract":"<jats:p>\n                    Biocatalysis harnesses enzymes to make valuable products. This green technology is used in countless applications from bench scale to industrial production and allows practitioners to access complex organic molecules, often with fewer synthetic steps and reduced waste. The last decade has seen an explosion in the development of experimental and computational tools to tailor enzymatic properties, equipping enzyme engineers with the ability to create biocatalysts that perform reactions not present in nature. By using (chemo)-enzymatic synthesis routes or orchestrating intricate enzyme cascades, scientists can synthesize elaborate targets ranging from DNA and complex pharmaceuticals to starch made in vitro from CO\n                    <jats:sub>2<\/jats:sub>\n                    -derived methanol. In addition, new chemistries have emerged through the combination of biocatalysis with transition metal catalysis, photocatalysis, and electrocatalysis. This review highlights recent key developments, identifies current limitations, and provides a future prospect for this rapidly developing technology.\n                  <\/jats:p>","DOI":"10.1126\/science.adh8615","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T14:01:20Z","timestamp":1700748080000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":740,"title":["From nature to industry: Harnessing enzymes for biocatalysis"],"prefix":"10.1126","volume":"382","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5997-1616","authenticated-orcid":true,"given":"R.","family":"Buller","sequence":"first","affiliation":[{"name":"Competence Center for Biocatalysis, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, 8820 W\u00e4denswil, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1043-0162","authenticated-orcid":true,"given":"S.","family":"Lutz","sequence":"additional","affiliation":[{"name":"Codexis Incorporated, Redwood City, CA 94063, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3570-2411","authenticated-orcid":true,"given":"R. J.","family":"Kazlauskas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology and Biophysics, Biotechnology Institute, University of Minnesota, Saint Paul, MN 55108, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4809-1066","authenticated-orcid":true,"given":"R.","family":"Snajdrova","sequence":"additional","affiliation":[{"name":"Novartis Institutes for BioMedical Research, Global Discovery Chemistry, 4056 Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9807-6315","authenticated-orcid":true,"given":"J. C.","family":"Moore","sequence":"additional","affiliation":[{"name":"MRL, Merck &amp; Co., Rahway, NJ 07065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0685-2696","authenticated-orcid":true,"given":"U. T.","family":"Bornscheuer","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Dept. of Biotechnology and Enzyme Catalysis, Greifswald University, Greifswald, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11117"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-019-0385-5"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-021-00044-z"},{"key":"e_1_3_2_5_2","unstructured":"K. Buchholz V. Kasche U. T. Bornscheuer Biocatalysts and Enzyme Technology . (Wiley-VCH ed. 2 2012)."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"A. S. Bommarius B. R. Riebel Biocatalysis . (Wiley-VCH 2004).","DOI":"10.1002\/3527602364"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trechm.2022.03.002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24621"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1720115115"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1100"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1080"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04964-5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2015.04.015"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-022-02652-8"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/marc.200500410"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35237-x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06725-11"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.checat.2022.09.011"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-018-0053-0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201907245"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-27999-1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b04691"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.2c00115"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b08325"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/225563a0"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.22562"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188934"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202004449"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201411901"},{"key":"e_1_3_2_30_2","unstructured":"O. Alvizo M. A. Mayo J. C. Moore US Patent US10370648B2. (2014)."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep35716"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201409470"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2017.06.005"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.0c00591"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0808-5"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c03428"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06027-2"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-023-00979-4"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abk1603"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-023-00986-5"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c04334"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crchbi.2021.100017"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr0204348"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-018-1317-y"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00767"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16099-7"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw1143"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c03607"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05167-1"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-022-01083-z"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-018-0059-y"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adg2420"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04456-z"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2596"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05696-3"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(01)90461-7"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41570-022-00456-9"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi0263"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4173"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201800658"},{"key":"e_1_3_2_61_2","unstructured":"C. Ching S. M. Forget M. A. Knight M. Krawczyk N. Mahmoodi M. A. Mayo M. G. Miller J. Vroom Codexis Inc. World Intellectual Property Organization Patent Application WO 2023\/069865 US Patent Application PCT\/US2022\/078071 (2023)."},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40259-022-00549-3"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.add5892"},{"key":"e_1_3_2_64_2","unstructured":"D. J. Wiegand J. Rittichier E. Meyer H. Lee N. J. Conway D. Ahlstedt Z. Yurtsever D. Rainone E.  Kuru G. M. Church A platform for controlled template-independent enzymatic synthesis of RNA oligonucleotides and therapeutics. bioRxiv 2023.06.2029.547106 [Preprint] (2023). 10.1101\/2023.06.29.547106 2023.06.2029.547106"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2918"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202100507"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2023.09.013"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202006648"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS01575J"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0496-458"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.7b00359"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0186-3"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-022-00119-5"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg8712"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06032"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00910"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-019-0340-5"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-019-0505-4"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c12004"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201610129"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b07029"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b05129"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacsau.1c00145"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b12621"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b02272"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-018-0105-6"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-021-00833-9"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba6794"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.2c01037"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05335-3"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj8754"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.ade2574"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacsau.3c00188"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01685-y"},{"key":"e_1_3_2_96_2","unstructured":"G. Corso H. St\u00e4rk B. Jing R. Barzilay T. Jaakkola DiffDock: Diffusion Steps Twists and Turns for Molecular Docking. arXiv:2210.01776v2 [q-bio.BM] (2023)."},{"key":"e_1_3_2_97_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1010483"},{"key":"e_1_3_2_98_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4426"},{"key":"e_1_3_2_99_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04184-w"},{"key":"e_1_3_2_100_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.add2187"},{"key":"e_1_3_2_101_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn2100"},{"key":"e_1_3_2_102_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.add1964"},{"key":"e_1_3_2_103_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.062353106"},{"key":"e_1_3_2_104_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1089427"},{"key":"e_1_3_2_105_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21086"},{"key":"e_1_3_2_106_2","doi-asserted-by":"crossref","unstructured":"R. Rombach A. Blattmann D. Lorenz P. Esser B. Ommer High-Resolution Image Synthesis with Latent Diffusion Models. arXiv:2112.10752v2 [cs.CV] (2021).","DOI":"10.1109\/CVPR52688.2022.01042"},{"key":"e_1_3_2_107_2","doi-asserted-by":"crossref","unstructured":"J. L. Watson D. Juergens N. R. Bennett B. L. Trippe J. Yim H. E. Eisenach W. Ahern A. J. Borst R. J. Ragotte L. F. Milles B. I. M. Wicky N. Hanikel S. J. Pellock A. Courbet W. Sheffler J. Wang P. Venkatesh I. Sappington S. V\u00e1zquez Torres A.  Lauko V. De Bortoli E. Mathieu R. Barzilay T. S. Jaakkola F. DiMaio M. Baek D. Baker Broadly applicable and accurate protein design by integrating structure prediction networks and diffusion generative models. bioRxiv2022.2012.2009.519842 [Preprint] (2022). https:\/\/doi.org\/10.1101\/2022.12.09.519842. 2022.2012.2009.519842","DOI":"10.1101\/2022.12.09.519842"},{"key":"e_1_3_2_108_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01618-2"},{"key":"e_1_3_2_109_2","doi-asserted-by":"publisher","DOI":"10.1038\/351491a0"},{"key":"e_1_3_2_110_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201812532"},{"key":"e_1_3_2_111_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23303-9"},{"key":"e_1_3_2_112_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adf2465"},{"key":"e_1_3_2_113_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01763-1"},{"key":"e_1_3_2_114_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2104878118"},{"key":"e_1_3_2_115_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0496-6"},{"key":"e_1_3_2_116_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1901979116"},{"key":"e_1_3_2_117_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.1c02786"},{"key":"e_1_3_2_118_2","doi-asserted-by":"publisher","DOI":"10.1002\/wcms.1502"},{"key":"e_1_3_2_119_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.1c03950"},{"key":"e_1_3_2_120_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-017-0010-4"},{"key":"e_1_3_2_121_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04422-9"},{"key":"e_1_3_2_122_2","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.1c00608"},{"key":"e_1_3_2_123_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay8484"},{"key":"e_1_3_2_124_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201611063"},{"key":"e_1_3_2_125_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn2009"},{"key":"e_1_3_2_126_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aah5237"},{"key":"e_1_3_2_127_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abh4049"},{"key":"e_1_3_2_128_2","unstructured":"E. Eichhorn B. Schilling D. Wahler L. Fourage E. Locher Givaudan SA World Intellectual Property Organization Patent WO 2016\/170099 (2015)."},{"key":"e_1_3_2_129_2","doi-asserted-by":"publisher","DOI":"10.2533\/chimia.2023.384"},{"key":"e_1_3_2_130_2","unstructured":"E. Eichhorn C. Ullmann Givaudan SA World Intellectual Property Organization Patent Application WO 2021\/110848 (2019)."},{"key":"e_1_3_2_131_2","unstructured":"S. McKenna Y. Tan M. J. Smith R. J. Fox D. T. George C. L. Joe Bristol Myers Squibb World Intellectual Property Organization Patent Application WO 2023\/178061 (2023)."},{"key":"e_1_3_2_132_2","unstructured":"C. Ching D. Elgart S. Jenne L. L. Matzdorff J. Nazor M. Rohovie Z. M. Seibel Codexis World Intellectual Property Organization Patent Application WO 2023\/150568 (2023)."},{"key":"e_1_3_2_133_2","doi-asserted-by":"publisher","DOI":"10.1039\/CS9881700111"},{"key":"e_1_3_2_134_2","unstructured":"S. Warren P. Wyatt Organic Synthesis: The Disconnection Approach (Wiley-Blackwell ed. 2 2008)."},{"key":"e_1_3_2_135_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-020-00556-z"},{"key":"e_1_3_2_136_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201702235"},{"key":"e_1_3_2_137_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201612462"},{"key":"e_1_3_2_138_2","doi-asserted-by":"crossref","unstructured":"N. J. Turner L. Humphreys Biocatalysis in Organic Synthesis: The Retrosynthesis Approach (Royal Society of Chemistry 2018).","DOI":"10.1039\/9781839169113"},{"key":"e_1_3_2_139_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1235"},{"key":"e_1_3_2_140_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-022-00909-w"},{"key":"e_1_3_2_141_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.580"},{"key":"e_1_3_2_142_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00129-0"},{"key":"e_1_3_2_143_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacsau.2c00617"},{"key":"e_1_3_2_144_2","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.202300127"},{"key":"e_1_3_2_145_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.0c02291"},{"key":"e_1_3_2_146_2","unstructured":"A. Crameri M. L. Hill S. L. Lovelock M. Schober D. G. Tew P. J. Thomas Glaxo Smith Kline World Intellectual Property Organization Patent WO 2018\/011067 A2 (2018)."},{"key":"e_1_3_2_147_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.08.038"},{"key":"e_1_3_2_148_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2022.153696"},{"key":"e_1_3_2_149_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00644"},{"key":"e_1_3_2_150_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2149-4"},{"key":"e_1_3_2_151_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04599-z"},{"key":"e_1_3_2_152_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202216220"},{"key":"e_1_3_2_153_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.oprd.8b00232"},{"key":"e_1_3_2_154_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00454"},{"key":"e_1_3_2_155_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00416"},{"key":"e_1_3_2_156_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1190239"},{"key":"e_1_3_2_157_2","doi-asserted-by":"crossref","unstructured":"A. Crossley R. Crawshaw F. Hardy L. Johannissen T. Lister E. G\u00e9rard Z. Birch-Price R. Obexer S. Hay A. Green A non-canonical nucleophile unlocks a new mechanistic pathway in a designed enzyme. Research Square [Preprint] (2023). https:\/\/doi.org\/10.21203\/rs.3.rs-2922796\/v1","DOI":"10.21203\/rs.3.rs-2922796\/v1"},{"key":"e_1_3_2_158_2","doi-asserted-by":"crossref","unstructured":"W. C. Hallows K. Skvorak N. Agard N. Kruse X. Zhang Y. Zhu R. C. Botham C. Chng C. Shukla J. Lao M. Miller A. Sero J. Viduya M. H. A. Ismaili K. McCluskie R. Schiffmann A. P. Silverman J.-S. Shen G. W. Huisman Optimizing human \u03b1-galactosidase for treatment of Fabry disease. Sci. Rep. 13 4748 (2023). 10.1038\/s41598-023-31777-436959353","DOI":"10.1038\/s41598-023-31777-4"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.adh8615","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T18:37:27Z","timestamp":1705343847000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.adh8615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,24]]},"references-count":157,"journal-issue":{"issue":"6673","published-print":{"date-parts":[[2023,11,24]]}},"alternative-id":["10.1126\/science.adh8615"],"URL":"https:\/\/doi.org\/10.1126\/science.adh8615","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,24]]},"assertion":[{"value":"2023-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadh8615"}}