{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:43:18Z","timestamp":1790790198139,"version":"4.1.0"},"reference-count":77,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6693","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Science"],"published-print":{"date-parts":[[2024,4,19]]},"abstract":"<jats:p>\n                    Despite an increasingly detailed picture of the molecular mechanisms of bacteriophage (phage)\u2013bacterial interactions, we lack an understanding of how these interactions evolve and impact disease within patients. In this work, we report a year-long, nationwide study of diarrheal disease patients in Bangladesh. Among cholera patients, we quantified\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    (prey) and its virulent phages (predators) using metagenomics and quantitative polymerase chain reaction while accounting for antibiotic exposure using quantitative mass spectrometry. Virulent phage (ICP1) and antibiotics suppressed\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    to varying degrees and were inversely associated with severe dehydration depending on resistance mechanisms. In the absence of antiphage defenses, predation was \u201ceffective,\u201d with a high predator:prey ratio that correlated with increased genetic diversity among the prey. In the presence of antiphage defenses, predation was \u201cineffective,\u201d with a lower predator:prey ratio that correlated with increased genetic diversity among the predators. Phage-bacteria coevolution within patients should therefore be considered in the deployment of phage-based therapies and diagnostics.\n                  <\/jats:p>","DOI":"10.1126\/science.adj3166","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T13:59:28Z","timestamp":1713448768000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":41,"title":["Phage predation, disease severity, and pathogen genetic diversity in cholera patients"],"prefix":"10.1126","volume":"384","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8793-7389","authenticated-orcid":true,"given":"Na\u00efma","family":"Madi","sequence":"first","affiliation":[{"name":"Department of Microbiology &amp; Immunology, McGill University, Montr\u00e9al, QC, Canada."},{"name":"McGill Genome Centre, McGill University, Montr\u00e9al, QC, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilee T.","family":"Cato","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Environmental and Global Health, University of Florida, Gainesville, FL, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md.","family":"Abu Sayeed","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Environmental and Global Health, University of Florida, Gainesville, FL, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashton","family":"Creasy-Marrazzo","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Environmental and Global Health, University of Florida, Gainesville, FL, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8522-6134","authenticated-orcid":true,"given":"Aline","family":"Cu\u00e9nod","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Immunology, McGill University, Montr\u00e9al, QC, Canada."},{"name":"McGill Genome Centre, McGill University, Montr\u00e9al, QC, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamrul","family":"Islam","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division (IDD) &amp; Nutrition and Clinical Services Division (NCSD), International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Imam Ul","family":"Khabir","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division (IDD) &amp; Nutrition and Clinical Services Division (NCSD), International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3755-4763","authenticated-orcid":true,"given":"Md. 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