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Here we introduce Green enhanced Nano-lantern (GeNL), a genetically encoded bioluminescent oxygen indicator for\n            <jats:italic>P<\/jats:italic>\n            <jats:sc>o<\/jats:sc>\n            <jats:sub>2<\/jats:sub>\n            imaging. In awake behaving mice, we uncover the existence of spontaneous, spatially defined \u201chypoxic pockets\u201d and demonstrate their linkage to the abrogation of local capillary flow. Exercise reduced the burden of hypoxic pockets by 52% compared with rest. The study provides insight into cortical oxygen dynamics in awake behaving animals and concurrently establishes a tool to delineate the importance of oxygen tension in physiological processes and neurological diseases.\n          <\/jats:p>","DOI":"10.1126\/science.adn1011","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T17:59:47Z","timestamp":1711648787000},"page":"1471-1478","update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":82,"title":["Oxygen imaging of hypoxic pockets in the mouse cerebral cortex"],"prefix":"10.1126","volume":"383","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6411-0909","authenticated-orcid":true,"given":"Felix R. 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