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Inoculation of 1.6 \u00d7 10<jats:sup>3<\/jats:sup>colony forming units (CFUs) of<jats:italic>Rosenbergiella_<\/jats:italic>YN46 into<jats:italic>A. albopictus<\/jats:italic>mosquitoes effectively prevents viral infection. Mechanistically, this bacterium secretes glucose dehydrogenase (<jats:italic>Ry<\/jats:italic>GDH), which acidifies the gut lumen of fed mosquitoes, causing irreversible conformational changes in the flavivirus envelope protein that prevent viral entry into cells. In semifield conditions,<jats:italic>Rosenbergiella_<\/jats:italic>YN46 exhibits effective transstadial transmission in field mosquitoes, which blocks transmission of dengue virus by newly emerged adult mosquitoes. 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