{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:47:54Z","timestamp":1792010874870,"version":"4.4.0"},"reference-count":59,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6727","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Science"],"published-print":{"date-parts":[[2024,12,13]]},"abstract":"<jats:p>\n                    Emotional experiences often evoke neural plasticity that supports adaptive changes in behavior, including maladaptive plasticity associated with mood and substance use disorders. These adaptations are supported in part by experience-dependent activation of immediate-early response genes, such as\n                    <jats:italic>Npas4<\/jats:italic>\n                    (neuronal PAS domain protein 4). Here we show that a conserved long noncoding enhancer RNA (lnc-eRNA), transcribed from an activity-sensitive enhancer, produces DNA:RNA hybrid R-loop structures that support three-dimensional chromatin looping between enhancer and proximal promoter and rapid\n                    <jats:italic>Npas4<\/jats:italic>\n                    gene induction. Furthermore, in mouse models,\n                    <jats:italic>Npas4<\/jats:italic>\n                    lnc-eRNA and its R-loop are required for the development of behavioral adaptations produced by chronic psychosocial stress or cocaine exposure, revealing a potential role for this regulatory mechanism in the transmission of emotional experiences.\n                  <\/jats:p>","DOI":"10.1126\/science.adp1562","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T14:00:41Z","timestamp":1734012041000},"page":"1282-1289","update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":45,"title":["A long noncoding eRNA forms R-loops to shape emotional experience\u2013induced behavioral adaptation"],"prefix":"10.1126","volume":"386","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8141-5344","authenticated-orcid":true,"given":"Rose Marie","family":"Akiki","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."},{"name":"Medical Scientist Training Program, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5086-2557","authenticated-orcid":true,"given":"Rebecca G.","family":"Cornbrooks","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Magami","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0811-8416","authenticated-orcid":true,"given":"Alain","family":"Greige","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."},{"name":"Medical Scientist Training Program, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten K.","family":"Snyder","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1874-7738","authenticated-orcid":true,"given":"Daniel J.","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."},{"name":"Medical Scientist Training Program, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Claire","family":"Herrington","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Mace","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6997-7221","authenticated-orcid":true,"given":"Kyle","family":"Blidy","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuya","family":"Koike","sequence":"additional","affiliation":[{"name":"Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Berto","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5472-3296","authenticated-orcid":true,"given":"Christopher W.","family":"Cowan","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6356-2463","authenticated-orcid":true,"given":"Makoto","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005744"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.psych.60.110707.163539"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.10.013"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11860"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.75631"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.09.015"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2017.11.009"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/543183a"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4170"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2020.543893"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2020.03.023"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00377"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09033"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.08.023"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-020-00640-3"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0206-3"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05985-9"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.242990.114"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.13.5764"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1208049"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.058"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07319"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2016.02.003"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.09.023"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0932-1"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0495-18.2019"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2020.5906"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2477-7_7"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.10.008"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R117.813485"},{"key":"e_1_3_2_32_2","first-page":"59","volume-title":"Methods of Behavior Analysis in Neuroscience","author":"Prus A. J.","year":"2009","unstructured":"A. J. Prus, J. R. James, J. A. Rosecrans, \u201cConditioned place preference\u201d in Methods of Behavior Analysis in Neuroscience, J. J. Buccafusco, Ed. (CRC Press, ed. 2, 2009), pp. 59\u201376.","edition":"2"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1037\/bne0000418"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-018-0011-z"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103388"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07672"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1146"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867325666171229132619"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-018-0306-6"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2016.10.002"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.025"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1571-12.2013"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1103"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.032"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2022.09.012"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05711-7"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-023-01026-2"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3871"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0013-16.2016"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7774-1_5"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1731-10.2010"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq033"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.3791\/62455"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067799"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.09.018"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.361"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/gbb.12561"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.adp1562","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T14:01:23Z","timestamp":1734012083000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.adp1562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,13]]},"references-count":59,"journal-issue":{"issue":"6727","published-print":{"date-parts":[[2024,12,13]]}},"alternative-id":["10.1126\/science.adp1562"],"URL":"https:\/\/doi.org\/10.1126\/science.adp1562","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,13]]},"assertion":[{"value":"2024-03-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}