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We generated more than 175,000 transposon insertions in the\n                    <jats:italic>Plasmodium knowlesi<\/jats:italic>\n                    genome, averaging an insertion every 138 base pairs, and used this \u201csupersaturation\u201d mutagenesis to score essentiality for 98% of genes. The density of mutations allowed mapping of putative essential domains within genes, providing a completely new level of genome annotation for any\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species. Although gene essentiality was largely conserved across\n                    <jats:italic>P. knowlesi<\/jats:italic>\n                    ,\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    , and rodent malaria model\n                    <jats:italic>Plasmodium berghei<\/jats:italic>\n                    , a large number of shared genes are differentially essential, revealing species-specific adaptations. Our results indicated that\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    essential gene evolution was conditionally linked to adaptive rewiring of metabolic networks for different hosts.\n                  <\/jats:p>","DOI":"10.1126\/science.adq7347","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T13:59:31Z","timestamp":1738850371000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":35,"title":["Supersaturation mutagenesis reveals adaptive rewiring of essential genes among malaria parasites"],"prefix":"10.1126","volume":"387","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3033-2212","authenticated-orcid":true,"given":"Jenna","family":"Oberstaller","sequence":"first","affiliation":[{"name":"Center for Global Health and Interdisciplinary Research and USF Genomics Program, College of Public Health, University of South Florida, Tampa, FL, 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