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These bnAb precursors, which target the CD4 receptor binding site, had undergone somatic hypermutation characteristic of the VRC01-class. A subset of isolated VRC01-class monoclonal antibodies neutralized wild-type pseudoviruses and was structurally extremely similar to bnAb VRC01. These results further support germline-targeting approaches for human HIV vaccine design and demonstrate atomic-level manipulation of B cell responses with rational vaccine design.\n          <\/jats:p>","DOI":"10.1126\/science.adv5572","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T13:59:11Z","timestamp":1747317551000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":59,"title":["Precise targeting of HIV broadly neutralizing antibody precursors in humans"],"prefix":"10.1126","volume":"389","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9580-157X","authenticated-orcid":true,"given":"Tom G.","family":"Caniels","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infection Prevention, Amsterdam UMC, location AMC, Amsterdam, Netherlands."},{"name":"Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1981-9054","authenticated-orcid":true,"given":"Madhu","family":"Prabhakaran","sequence":"additional","affiliation":[{"name":"Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9695-8138","authenticated-orcid":true,"given":"Gabriel","family":"Ozorowski","sequence":"additional","affiliation":[{"name":"Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0993-4009","authenticated-orcid":true,"given":"Kellie J.","family":"MacPhee","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6015-9167","authenticated-orcid":true,"given":"Weiwei","family":"Wu","sequence":"additional","affiliation":[{"name":"Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6373-9302","authenticated-orcid":true,"given":"Karlijn","family":"van der Straten","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infection Prevention, Amsterdam UMC, location AMC, Amsterdam, Netherlands."},{"name":"Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8549-9602","authenticated-orcid":true,"given":"Sashank","family":"Agrawal","sequence":"additional","affiliation":[{"name":"Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Derking","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infection Prevention, Amsterdam UMC, location AMC, Amsterdam, Netherlands."},{"name":"Amsterdam Institute for Immunology and Infectious Diseases, Amsterdam, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4695-0628","authenticated-orcid":true,"given":"Emma I. 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