{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:16:05Z","timestamp":1791432965312,"version":"4.3.3"},"reference-count":48,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6772","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Science"],"published-print":{"date-parts":[[2025,10,30]]},"abstract":"<jats:p>\n                    Recycling thermoset polyurethanes is hindered by their cross-linked structures and chemically stable urethane bonds. Although chemo-enzymatic approaches offer promise, known urethanases remain inefficient under industrial glycolysis conditions. Here, we present GRASE [graph neural network (GNN)\u2013based recommendation of active and stable enzymes], a GNN-based framework that integrates self-supervised and supervised learning to identify efficient, glycolysis-compatible urethanases. Among these,\n                    <jats:italic toggle=\"yes\">Ab<\/jats:italic>\n                    PURase exhibited two orders of magnitude greater activity than previously known enzymes in 6 molar diethylene glycol, enabling near-complete depolymerization of commercial polyurethane at kilogram scale within 8 hours. Structural analysis revealed that a tightly packed hydrophobic core and proline-stabilized lid loop may confer\n                    <jats:italic toggle=\"yes\">Ab<\/jats:italic>\n                    PURase\u2019s stability and efficiency in harsh solvents. This work highlights how deep learning accelerates the discovery of biocatalysts with industrial potential and addresses a critical barrier in polyurethane recycling.\n                  <\/jats:p>","DOI":"10.1126\/science.adw4487","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T18:01:37Z","timestamp":1761847297000},"page":"503-509","update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":44,"title":["Glycolysis-compatible urethanases for polyurethane recycling"],"prefix":"10.1126","volume":"390","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5337-141X","authenticated-orcid":true,"given":"Yanchun","family":"Chen","sequence":"first","affiliation":[{"name":"AIM Center, College of Life Sciences and Technology, Beijing University of Chemical Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3456-0332","authenticated-orcid":true,"given":"Jinyuan","family":"Sun","sequence":"additional","affiliation":[{"name":"AIM Center, College of Life Sciences and Technology, Beijing University of Chemical Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China."},{"name":"University of Chinese Academy of Sciences, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9063-4006","authenticated-orcid":true,"given":"Kelun","family":"Shi","sequence":"additional","affiliation":[{"name":"AIM Center, College of Life Sciences and Technology, Beijing University of Chemical Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China."},{"name":"University of Chinese Academy of Sciences, Beijing, 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Chinese Academy of Sciences, Beijing, China."},{"name":"State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9464-2860","authenticated-orcid":true,"given":"Xiaomeng","family":"Liu","sequence":"additional","affiliation":[{"name":"AIM Center, College of Life Sciences and Technology, Beijing University of Chemical Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China."},{"name":"University of Chinese Academy of Sciences, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5957-9617","authenticated-orcid":true,"given":"Xinying","family":"Xie","sequence":"additional","affiliation":[{"name":"AIM Center, College of Life Sciences and Technology, Beijing University of Chemical Technology, Institute of Microbiology, Chinese Academy of 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China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6835-4962","authenticated-orcid":true,"given":"Jinwei","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7036-8917","authenticated-orcid":true,"given":"Wenyu","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Biological Sciences, China Agricultural University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2147-5879","authenticated-orcid":true,"given":"Yinglu","family":"Cui","sequence":"additional","affiliation":[{"name":"AIM Center, College of Life Sciences and Technology, Beijing University of Chemical Technology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China."},{"name":"State 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