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We describe an intranasal liposomal formulation combining toll-like receptor 4 and 7\/8 ligands with a model antigen, ovalbumin, which provided broad, durable protection in mice for at least 3 months against infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    . In addition, the vaccine protected mice from other viruses (SARS-CoV-2, SARS, SHC014 coronavirus), bacteria (\n                    <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                    ), and allergens. Protection was mediated by persistent ovalbumin-specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    memory T cells that imprinted alveolar macrophages (AMs), enhancing antigen presentation and antiviral immunity. Following infection, vaccinated mice mounted rapid pathogen-specific T cell and antibody responses and formed ectopic lymphoid structures in the lung. These results reveal a class of \u201cuniversal vaccines\u201d against diverse respiratory threats.\n                  <\/jats:p>","DOI":"10.1126\/science.aea1260","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T19:03:38Z","timestamp":1771527818000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":30,"title":["Mucosal vaccination in mice provides protection from diverse respiratory threats"],"prefix":"10.1126","volume":"392","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4629-8956","authenticated-orcid":true,"given":"Haibo","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford University, Stanford, CA, 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