{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:58:18Z","timestamp":1785437898251,"version":"3.56.0"},"reference-count":50,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"6810","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32341016"],"award-info":[{"award-number":["32341016"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tsinghua University Initiative Scientific Research Program","award":["20241080049"],"award-info":[{"award-number":["20241080049"]}]},{"name":"Tsinghua University Initiative Scientific Research Program","award":["20251080093"],"award-info":[{"award-number":["20251080093"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Science"],"published-print":{"date-parts":[[2026,7,30]]},"abstract":"<jats:p>Collagen, a fundamental constituent of the extracellular matrix, has long remained elusive to high-resolution structural characterization. Using a tailored system and optimized cryo\u2013electron microscopy processing for long-period filaments, we determined the structure of native collagen fibrils from the porcine vitreous body, with local resolutions extending from 2.6 to 7 angstroms. Each 67-nanometer periodic unit contains type II, V\/XI, and IX collagen triple helices together with opticin, at a stoichiometry of 8:4:4:4, which reveals their detailed higher-order molecular packing. Abundant galactose-glucose disaccharides modify hydroxylysine residues in conserved -glycine-X-hydroxylysine- motifs, mediating fibril packing and structural stability. Our structure uncovers the glycan-mediated assembly principle of collagen fibrils and clarifies the structure-function basis of collagens in the vitreous body.<\/jats:p>","DOI":"10.1126\/science.aec2906","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:01:01Z","timestamp":1785434461000},"page":"509-513","update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["The molecular architecture of mammalian vitreous body collagen fibrils"],"prefix":"10.1126","volume":"393","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9088-1395","authenticated-orcid":true,"given":"Xinyao","family":"Lou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structures, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6141-2293","authenticated-orcid":true,"given":"Ye","family":"Cong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structures, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7050-8284","authenticated-orcid":true,"given":"Yuqian","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structures, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yazhao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structures, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4080-3214","authenticated-orcid":true,"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Technology Center for Protein Sciences, School of Life Sciences, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9338-8048","authenticated-orcid":true,"given":"Chuangye","family":"Yan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structures, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrp.2021.100515"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2003.08.002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197201272860406"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.068726"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-49674-0_14"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(73)90961-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(62)90295-4"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312807200"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/bse0520113"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(58)90053-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99789-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14197"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99529-3"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2003.11.004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep37831"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004978"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R600025200"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/219157a0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-416617-2.00009-6"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502718103"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/174269c0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/177710b0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7695699"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.3.2123"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2003.031989"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025383913444"},{"key":"e_1_3_2_28_2","first-page":"1540","article-title":"Collagens of the bovine vitreous","volume":"32","author":"Seery C. M.","year":"1991","unstructured":"C. M. Seery, P. F. Davison, Collagens of the bovine vitreous. Invest. Ophthalmol. Vis. Sci. 32, 1540\u20131550 (1991). 2016136","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2990497"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1996.159"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)77922-8"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50048-X"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3140327"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.5.1181"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1992.120"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/58\/11\/R37"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41433-024-02969-7"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(99)00016-6"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.12.4044"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(98)90020-8"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)67713-6"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2005.01.002"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4169"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.04.033"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4792"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07215-4"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1609\/aaai.v33i01.33011230"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798318006551"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319011471"}],"container-title":["Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/science.aec2906","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:02:03Z","timestamp":1785434523000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/science.aec2906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":50,"journal-issue":{"issue":"6810","published-print":{"date-parts":[[2026,7,30]]}},"alternative-id":["10.1126\/science.aec2906"],"URL":"https:\/\/doi.org\/10.1126\/science.aec2906","relation":{},"ISSN":["0036-8075","1095-9203"],"issn-type":[{"value":"0036-8075","type":"print"},{"value":"1095-9203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]},"assertion":[{"value":"2025-09-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}