{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:54:01Z","timestamp":1790952841650,"version":"4.1.0"},"reference-count":56,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"73","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Immunol."],"published-print":{"date-parts":[[2022,7,29]]},"abstract":"<jats:p>\n            T helper 17 (T\n            <jats:sub>H<\/jats:sub>\n            17) cells located at the Peyer\u2019s patch (PP) inductive site and at the lamina propria effector site of the intestinal immune system are responsive to both pathogenic and commensal bacteria. Their plasticity to convert into follicular helper T (T\n            <jats:sub>FH<\/jats:sub>\n            ) cells has been proposed to be central to gut immunoglobulin A (IgA) responses. Here, we used an IL-17A fate reporter mouse and an MHC-II tetramer to analyze antigen-specific CD4\n            <jats:sup>+<\/jats:sup>\n            T cell subsets and isolate them for single-cell RNA sequencing after oral immunization with cholera toxin and ovalbumin. We found a T\n            <jats:sub>FH<\/jats:sub>\n            -dominated response with only rare antigen-specific T\n            <jats:sub>H<\/jats:sub>\n            17 cells (&lt;8%) in the PP. A clonotypic analysis provided little support that clonotypes were shared between T\n            <jats:sub>FH<\/jats:sub>\n            and T\n            <jats:sub>H<\/jats:sub>\n            17 cells, arguing against T\n            <jats:sub>H<\/jats:sub>\n            17 plasticity as a major contributor to T\n            <jats:sub>FH<\/jats:sub>\n            differentiation. Two mouse models of T\n            <jats:sub>H<\/jats:sub>\n            17 deficiency confirmed that gut IgA responses to oral immunization do not require T\n            <jats:sub>H<\/jats:sub>\n            17 cells, with\n            <jats:italic>\n              CD4\n              <jats:sup>Cre<\/jats:sup>\n              Rorc\n              <jats:sup>fl\/fl<\/jats:sup>\n            <\/jats:italic>\n            mice exhibiting normal germinal centers in PP and unperturbed total IgA production in the intestine.\n          <\/jats:p>","DOI":"10.1126\/sciimmunol.abc5500","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T17:55:34Z","timestamp":1656698134000},"source":"Crossref","is-referenced-by-count":25,"title":["Peyer\u2019s patch T\n            <sub>H<\/sub>\n            17 cells are dispensable for gut IgA responses to oral immunization"],"prefix":"10.1126","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5694-1315","authenticated-orcid":true,"given":"Inta","family":"Gribonika","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anneli","family":"Str\u00f6mberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9024-1240","authenticated-orcid":true,"given":"Cristina","family":"Lebrero-Fernandez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Sch\u00f6n","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8246-931X","authenticated-orcid":true,"given":"James","family":"Moon","sequence":"additional","affiliation":[{"name":"Center for Immunology and Inflammatory Diseases and Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7416-9819","authenticated-orcid":true,"given":"Mats","family":"Bemark","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden."},{"name":"Region V\u00e4stra G\u00f6taland, Sahlgrenska University Hospital, Department of Clinical Immunology and Transfusion Medicine, Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1155-4861","authenticated-orcid":true,"given":"Nils","family":"Lycke","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.12.003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032414-112056"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.05.004"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2552"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13014"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301668"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.14309\/crj.2018.56"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.2018026181"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052016"},{"key":"e_1_3_3_11_2","first-page":"301","article-title":"Strong adjuvant properties of cholera toxin on gut mucosal immune responses to orally presented antigens","volume":"59","author":"Lycke N.","year":"1986","unstructured":"N. Lycke, J. Holmgren, Strong adjuvant properties of cholera toxin on gut mucosal immune responses to orally presented antigens. Immunology 59, 301\u2013308 (1986).","journal-title":"Immunology"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2018.03.015"},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"2736","DOI":"10.4049\/jimmunol.132.6.2736","article-title":"Generalized systemic and mucosal immunity in mice after mucosal stimulation with cholera toxin","volume":"132","author":"Elson C. O.","year":"1984","unstructured":"C. O. Elson, W. Ealding, Generalized systemic and mucosal immunity in mice after mucosal stimulation with cholera toxin. J. Immunol. 132, 2736\u20132741 (1984).","journal-title":"J. Immunol."},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220915"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830261108"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002348107"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.05.016"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1169152"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14452"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1008247107"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf4822"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1716"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12704"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.12.022"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gdata.2015.07.009"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4314"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0904028"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.74"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024226"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3800"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00714"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812681106"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.62"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-019-0202-0"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.08.003"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aay2754"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.01.016"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3251"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3554"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.56"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00329"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06804-y"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12698"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7772"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200955"},{"key":"e_1_3_3_46_2","doi-asserted-by":"crossref","first-page":"188.110","DOI":"10.4049\/jimmunol.202.Supp.188.10","article-title":"B cell recruitment to the intestine critically depends on GATA3- and RORgt-mediated development of NKp46 negative innate lymphoid cells","volume":"202","author":"Zheng M.","year":"2019","unstructured":"M. Zheng, K. Mao, D. Li, D. Fang, J. Lyu, J. Zhu, B cell recruitment to the intestine critically depends on GATA3- and RORgt-mediated development of NKp46 negative innate lymphoid cells. J. Immunol. 202, 188.110 (2019).","journal-title":"J. Immunol."},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1194597"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.05.014"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.09.033"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700171"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.1.15-23.1994"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2014.111"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.116.197988"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-001-0408-6"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw377"}],"container-title":["Science Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciimmunol.abc5500","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T11:08:38Z","timestamp":1705403318000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciimmunol.abc5500"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,29]]},"references-count":56,"journal-issue":{"issue":"73","published-print":{"date-parts":[[2022,7,29]]}},"alternative-id":["10.1126\/sciimmunol.abc5500"],"URL":"https:\/\/doi.org\/10.1126\/sciimmunol.abc5500","relation":{},"ISSN":["2470-9468"],"issn-type":[{"value":"2470-9468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,29]]},"article-number":"eabc5500"}}