{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:22:23Z","timestamp":1790256143897,"version":"4.1.0"},"reference-count":46,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"67","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Immunol."],"published-print":{"date-parts":[[2022,1,28]]},"abstract":"<jats:p>\n                    Recent studies have established that memory B cells, largely thought to be circulatory in the blood, can take up long-term residency in inflamed tissues, analogous to widely described tissue-resident T cells. The dynamics of recruitment and retention of memory B cells to tissues and their immunological purpose remains unclear. Here, we characterized tissue-resident memory B cells (B\n                    <jats:sub>RM<\/jats:sub>\n                    ) that are stably maintained in the lungs of mice after pulmonary influenza infection. Influenza\n                    <jats:strike>-<\/jats:strike>\n                    specific B\n                    <jats:sub>RM<\/jats:sub>\n                    were localized within inducible bronchus-associated lymphoid tissues (iBALTs) and displayed transcriptional signatures distinct from classical memory B cells in the blood or spleen while showing partial overlap with memory B cells in lung-draining lymph nodes. We identified lung-resident markers, including elevated expression of CXCR3, CCR6, and CD69, on hemagglutinin (HA)\u2013 and nucleoprotein (NP)\u2013specific lung B\n                    <jats:sub>RM<\/jats:sub>\n                    . We found that CCR6 facilitates increased recruitment and\/or retention of B\n                    <jats:sub>RM<\/jats:sub>\n                    in lungs and differentiation into antibody-secreting cells upon recall. Although expression of CXCR3 and CCR6 was comparable in total and influenza-specific memory B cells isolated across tissues of human donors, CD69 expression was higher in memory B cells from lung and draining lymph nodes of human organ donors relative to splenic and PBMC-derived populations, indicating that mechanisms underpinning B\n                    <jats:sub>RM<\/jats:sub>\n                    localization may be evolutionarily conserved. Last, we demonstrate that human memory B cells in lungs are transcriptionally distinct to populations in lung-draining lymph nodes or PBMCs. These data suggest that B\n                    <jats:sub>RM<\/jats:sub>\n                    may constitute a discrete component of B cell immunity, positioned at the lung mucosa for rapid humoral response against respiratory viral infections.\n                  <\/jats:p>","DOI":"10.1126\/sciimmunol.abf5314","type":"journal-article","created":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T14:10:05Z","timestamp":1643379005000},"source":"Crossref","is-referenced-by-count":108,"title":["Lung-resident memory B cells established after pulmonary influenza infection display distinct transcriptional and phenotypic profiles"],"prefix":"10.1126","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1064-3934","authenticated-orcid":true,"given":"Hyon-Xhi","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9072-1017","authenticated-orcid":true,"given":"Jennifer A.","family":"Juno","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7091-0048","authenticated-orcid":true,"given":"Robyn","family":"Esterbauer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4652-5609","authenticated-orcid":true,"given":"Hannah G.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."},{"name":"ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen M.","family":"Wragg","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7798-9182","authenticated-orcid":true,"given":"Penny","family":"Konstandopoulos","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheilajen","family":"Alcantara","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."},{"name":"ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Division of Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8415-5565","authenticated-orcid":true,"given":"Robert","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Surgery, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4285-1343","authenticated-orcid":true,"given":"Graham","family":"Starkey","sequence":"additional","affiliation":[{"name":"Department of Surgery, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boa Zhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1219-5362","authenticated-orcid":true,"given":"Osamu","family":"Yoshino","sequence":"additional","affiliation":[{"name":"Department of Surgery, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7702-3479","authenticated-orcid":true,"given":"Thomas","family":"Tiang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Lindsay","family":"Grayson","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Opdam","sequence":"additional","affiliation":[{"name":"DonateLife, The Australian Organ and Tissue Authority, Canberra, Australian Capital Territory 2601, Australia."},{"name":"Department of Intensive Care, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2313-2623","authenticated-orcid":true,"given":"Rohit","family":"D\u2019Costa","sequence":"additional","affiliation":[{"name":"DonateLife Victoria, Carlton, Victoria 3053, Australia."},{"name":"Intensive Care Unit, The Royal Melbourne Hospital, Parkville, Victoria 3050, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Vago","sequence":"additional","affiliation":[{"name":"Department of Surgery, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the Austin Liver Transplant Perfusionist Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8496-6632","authenticated-orcid":true,"given":"Laura K.","family":"Mackay","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5172-4728","authenticated-orcid":true,"given":"Claire L.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."},{"name":"Department of Infectious Diseases, Austin Health, Heidelberg, Victoria 3084, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9440-3884","authenticated-orcid":true,"given":"David","family":"Masopust","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Minnesota, Minneapolis, MN, USA."},{"name":"Center for Immunology, University of Minnesota, Minneapolis, MN, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5251-7835","authenticated-orcid":true,"given":"Joanna R.","family":"Groom","sequence":"additional","affiliation":[{"name":"Division of Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia."},{"name":"Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8539-4891","authenticated-orcid":true,"given":"Stephen J.","family":"Kent","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."},{"name":"ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, Australia."},{"name":"Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria 3004, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5593-9387","authenticated-orcid":true,"given":"Adam K.","family":"Wheatley","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia."},{"name":"ARC Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Victoria 3010, 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Powell M. Milton A. Perry K. Santos drpowell\/degust 4.1.1 (Zenodo 2019); https:\/\/zenodo.org\/record\/3501067."}],"container-title":["Science Immunology"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T14:10:21Z","timestamp":1643379021000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciimmunol.abf5314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,28]]},"references-count":46,"journal-issue":{"issue":"67","published-print":{"date-parts":[[2022,1,28]]}},"alternative-id":["10.1126\/sciimmunol.abf5314"],"URL":"https:\/\/doi.org\/10.1126\/sciimmunol.abf5314","relation":{},"ISSN":["2470-9468"],"issn-type":[{"value":"2470-9468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,28]]},"article-number":"eabf5314"}}