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We single-cell\u2013profiled CD45\n            <jats:sup>+<\/jats:sup>\n            immune cell transcriptomes from skin samples of 31 patients (7 atopic dermatitis, 8 psoriasis vulgaris, 2 lichen planus (LP), 1 bullous pemphigoid (BP), 6 clinical\/histopathologically indeterminate rashes, and 7 healthy controls). Our data revealed active proliferative expansion of the T\n            <jats:sub>reg<\/jats:sub>\n            and Trm components and universal T cell exhaustion in human rashes, with a relative attenuation of antigen-presenting cells. Skin-resident memory T cells showed the greatest transcriptional dysregulation in both atopic dermatitis and psoriasis, whereas atopic dermatitis also demonstrated recurrent abnormalities in ILC and CD8\n            <jats:sup>+<\/jats:sup>\n            cytotoxic lymphocytes. Transcript signatures differentiating these rash types included genes previously implicated in T helper cell (T\n            <jats:sub>H<\/jats:sub>\n            2)\/T\n            <jats:sub>H<\/jats:sub>\n            17 diatheses, segregated in unbiased functional networks, and accurately identified disease class in untrained validation data sets. These gene signatures were able to classify clinicopathologically ambiguous rashes with diagnoses consistent with therapeutic response. Thus, we have defined major classes of human inflammatory skin disease at the molecular level and described a quantitative method to classify indeterminate instances of pathologic inflammation. To make this approach accessible to the scientific community, we created a proof-of-principle web interface (RashX), where scientists and clinicians can visualize their patient-level rash scRNA-seq\u2013derived data in the context of our T\n            <jats:sub>H<\/jats:sub>\n            2\/T\n            <jats:sub>H<\/jats:sub>\n            17 transcriptional framework.\n          <\/jats:p>","DOI":"10.1126\/sciimmunol.abl9165","type":"journal-article","created":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T17:57:15Z","timestamp":1650045435000},"source":"Crossref","is-referenced-by-count":170,"title":["Classification of human chronic inflammatory skin disease based on single-cell immune profiling"],"prefix":"10.1126","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3936-1834","authenticated-orcid":true,"given":"Yale","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Dermatology, The Second Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, ShaanXi 710004, P. 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