{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T23:56:45Z","timestamp":1792281405323,"version":"4.4.0"},"reference-count":70,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"84","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Immunol."],"published-print":{"date-parts":[[2023,6,30]]},"abstract":"<jats:p>Whereas the cellular and molecular features of human inflammatory skin diseases are well characterized, their tissue context and systemic impact remain poorly understood. We thus profiled human psoriasis (PsO) as a prototypic immune-mediated condition with a high predilection for extracutaneous involvement. Spatial transcriptomics (ST) analyses of 25 healthy, active lesion, and clinically uninvolved skin biopsies and integration with public single-cell transcriptomics data revealed marked differences in immune microniches between healthy and inflamed skin. Tissue-scale cartography further identified core disease features across all active lesions, including the emergence of an inflamed suprabasal epidermal state and the presence of B lymphocytes in lesional skin. Both lesional and distal nonlesional samples were stratified by skin disease severity and not by the presence of systemic disease. This segregation was driven by macrophage-, fibroblast-, and lymphatic-enriched spatial regions with gene signatures associated with metabolic dysfunction. Together, these findings suggest that mild and severe forms of PsO have distinct molecular features and that severe PsO may profoundly alter the cellular and metabolic composition of distal unaffected skin sites. In addition, our study provides a valuable resource for the research community to study spatial gene organization of healthy and inflamed human skin.<\/jats:p>","DOI":"10.1126\/sciimmunol.abq7991","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T17:58:24Z","timestamp":1685728704000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":129,"title":["Spatial transcriptomics stratifies psoriatic disease severity by emergent cellular ecosystems"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1704-9420","authenticated-orcid":true,"given":"Rochelle L.","family":"Castillo","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, NYU Langone Health, New York, NY 10016, USA."},{"name":"NYU Psoriatic Arthritis Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1470-6421","authenticated-orcid":true,"given":"Ikjot","family":"Sidhu","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."},{"name":"Applied Bioinformatics Laboratories, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4451-126X","authenticated-orcid":true,"given":"Igor","family":"Dolgalev","sequence":"additional","affiliation":[{"name":"Applied Bioinformatics Laboratories, NYU Langone Health, New York, NY 10016, USA."},{"name":"Translational Immunology Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tinyi","family":"Chu","sequence":"additional","affiliation":[{"name":"Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6065-9024","authenticated-orcid":true,"given":"Aleksandr","family":"Prystupa","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."},{"name":"Applied Bioinformatics Laboratories, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0191-6953","authenticated-orcid":true,"given":"Ipsita","family":"Subudhi","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1338-9847","authenticated-orcid":true,"given":"Di","family":"Yan","sequence":"additional","affiliation":[{"name":"Ronald O. Perelman Department of Dermatology, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4114-349X","authenticated-orcid":true,"given":"Piotr","family":"Konieczny","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3581-6264","authenticated-orcid":true,"given":"Brandon","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7119-8136","authenticated-orcid":true,"given":"Rebecca H.","family":"Haberman","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, NYU Langone Health, New York, NY 10016, USA."},{"name":"NYU Psoriatic Arthritis Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1718-0282","authenticated-orcid":true,"given":"Shanmugapriya","family":"Selvaraj","sequence":"additional","affiliation":[{"name":"Experimental Pathology Laboratory, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7611-8528","authenticated-orcid":true,"given":"Tomoe","family":"Shiomi","sequence":"additional","affiliation":[{"name":"Center for Biospecimen Research and Development, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhina","family":"Medina","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, NYU Langone Health, New York, NY 10016, USA."},{"name":"NYU Psoriatic Arthritis Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parvathy Vasudevanpillai","family":"Girija","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, NYU Langone Health, New York, NY 10016, USA."},{"name":"NYU Psoriatic Arthritis Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2836-6683","authenticated-orcid":true,"given":"Adriana","family":"Heguy","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."},{"name":"Genome Technology Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia A.","family":"Loomis","sequence":"additional","affiliation":[{"name":"Experimental Pathology Laboratory, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-6873","authenticated-orcid":true,"given":"Luis","family":"Chiriboga","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."},{"name":"Center for Biospecimen Research and Development, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2602-1219","authenticated-orcid":true,"given":"Christopher","family":"Ritchlin","sequence":"additional","affiliation":[{"name":"Allergy, Immunology and Rheumatology Division, Center of Musculoskeletal Research, University of Rochester Medical School, Rochester, NY 14642, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9268-2464","authenticated-orcid":true,"given":"Maria De La Luz","family":"Garcia-Hernandez","sequence":"additional","affiliation":[{"name":"Allergy, Immunology and Rheumatology Division, Center of Musculoskeletal Research, University of Rochester Medical School, Rochester, NY 14642, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6817-9439","authenticated-orcid":true,"given":"John","family":"Carucci","sequence":"additional","affiliation":[{"name":"Ronald O. Perelman Department of Dermatology, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane A.","family":"Meehan","sequence":"additional","affiliation":[{"name":"Ronald O. Perelman Department of Dermatology, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea L.","family":"Neimann","sequence":"additional","affiliation":[{"name":"Ronald O. Perelman Department of Dermatology, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0080-0812","authenticated-orcid":true,"given":"Johann E.","family":"Gudjonsson","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Michigan Medicine, University of Michigan, Ann Arbor, MI 48109, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1072-6994","authenticated-orcid":true,"given":"Jose U.","family":"Scher","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, NYU Langone Health, New York, NY 10016, USA."},{"name":"NYU Psoriatic Arthritis Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2216-5135","authenticated-orcid":true,"given":"Shruti","family":"Naik","sequence":"additional","affiliation":[{"name":"Department of Pathology, NYU Langone Health, New York, NY 10016, USA."},{"name":"Computational and Systems Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA."},{"name":"Perlmutter Cancer Center, NYU Langone Health, New York, NY 10016, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.04.032"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2016.05.012"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2017.05.010"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2010.370"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/acr.20627"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.296.14.1735"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2006.08.040"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2009.00745.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2005.03.046"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-201706"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.39494"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-019-0175-0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.09.015"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba6500"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.11.028"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf2403"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0392-8"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abg9302"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00922-4"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1349-1"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0619-0"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.08.056"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2012.15"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.222"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.07.019"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0667-5"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.04.005"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-022-00356-3"},{"key":"e_1_3_3_31_2","doi-asserted-by":"crossref","unstructured":"R. E. Niec T. Chu S. Gur-Cohen M. Schernthanner L. Hidalgo H. A. Pasolli R. P. Kataru B. J. Mehrara D. Pe\u2019er E. Fuchs A lymphatic-stem cell interactome regulates intestinal stem cell activity. bioRxiv 2022.01.29.478341 [Preprint]. 30 January 2022. https:\/\/doi.org\/10.1101\/2022.01.29.478341.","DOI":"10.1101\/2022.01.29.478341"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1080\/09546630310019382"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.28"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2100758"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1225152"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14052"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.19380"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.02.023"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0084-5"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24172"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.2298\/VSP0509613P"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.17689"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(18)30041-X"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1989.01670170053007"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.05.113"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/ced.12715"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-101721-062818"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431x20165374"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.1999.tb02021.x"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13027"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9122627"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-81588-8"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2015.11.003"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00540-z"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abn2263"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04919-3"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311000200"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.10.006"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.11.012"},{"key":"e_1_3_3_60_2","doi-asserted-by":"crossref","unstructured":"D. E. Johnson L. T. Williams in Advances in Cancer Research G. F. Vande Woude G. Klein Eds. (Academic Press 1992) vol. 60 pp. 1\u201341.","DOI":"10.1016\/S0065-230X(08)60821-0"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.04.021"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-6161-8"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800837"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700705"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.143.9.1153"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2009.00625.x"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2006.08.016"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200910126"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.07.024"},{"key":"e_1_3_3_70_2","first-page":"2122","article-title":"A step-by-step workflow for low-level analysis of single-cell RNA-seq data with Bioconductor","volume":"5","author":"Lun A. T.","year":"2016","unstructured":"A. T. Lun, D. J. McCarthy, J. C. Marioni, A step-by-step workflow for low-level analysis of single-cell RNA-seq data with Bioconductor. F1000Res. 5, 2122 (2016).","journal-title":"F1000Res."},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0336-3"}],"container-title":["Science Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciimmunol.abq7991","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T11:02:19Z","timestamp":1705402939000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciimmunol.abq7991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,30]]},"references-count":70,"journal-issue":{"issue":"84","published-print":{"date-parts":[[2023,6,30]]}},"alternative-id":["10.1126\/sciimmunol.abq7991"],"URL":"https:\/\/doi.org\/10.1126\/sciimmunol.abq7991","relation":{},"ISSN":["2470-9468"],"issn-type":[{"value":"2470-9468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,30]]},"assertion":[{"value":"2022-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eabq7991"}}