{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T23:47:59Z","timestamp":1789084079640,"version":"build-2803163510"},"reference-count":57,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"96","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Immunol."],"published-print":{"date-parts":[[2024,6,14]]},"abstract":"<jats:p>\n                    Expression of the long noncoding RNA (lncRNA) metastasis\u2013associated lung adenocarcinoma transcript 1 (\n                    <jats:italic>MALAT1<\/jats:italic>\n                    ) correlates with tumor progression and metastasis in many tumor types. However, the impact and mechanism of action by which\n                    <jats:italic>MALAT1<\/jats:italic>\n                    promotes metastatic disease remain elusive. Here, we used CRISPR activation (CRISPRa) to overexpress\n                    <jats:italic>MALAT1\/Malat1<\/jats:italic>\n                    in patient-derived lung adenocarcinoma (LUAD) cell lines and in the autochthonous K-ras\/p53 LUAD mouse model.\n                    <jats:italic>Malat1<\/jats:italic>\n                    overexpression was sufficient to promote the progression of LUAD to metastatic disease in mice. Overexpression of\n                    <jats:italic>MALAT1\/Malat1<\/jats:italic>\n                    enhanced cell mobility and promoted the recruitment of protumorigenic macrophages to the tumor microenvironment through paracrine secretion of CCL2\/Ccl2.\n                    <jats:italic>Ccl2<\/jats:italic>\n                    up-regulation was the result of increased global chromatin accessibility upon\n                    <jats:italic>Malat1<\/jats:italic>\n                    overexpression. Macrophage depletion and Ccl2 blockade counteracted the effects of\n                    <jats:italic>Malat1<\/jats:italic>\n                    overexpression. These data demonstrate that a single lncRNA can drive LUAD metastasis through reprogramming of the tumor microenvironment.\n                  <\/jats:p>","DOI":"10.1126\/sciimmunol.adh5462","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T14:01:11Z","timestamp":1718373671000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":47,"title":["Overexpression of\n                    <i>Malat1<\/i>\n                    drives metastasis through inflammatory reprogramming of the tumor microenvironment"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1956-0975","authenticated-orcid":true,"given":"Elena","family":"Martinez-Terroba","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06511, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0161-3277","authenticated-orcid":true,"given":"Leah M.","family":"Plasek-Hegde","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06511, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6973-3842","authenticated-orcid":true,"given":"Ioannis","family":"Chiotakakos","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06511, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4613-4040","authenticated-orcid":true,"given":"Vincent","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5847-9371","authenticated-orcid":true,"given":"Fernando J.","family":"de Miguel","sequence":"additional","affiliation":[{"name":"Yale Cancer Center, Yale University, New Haven, CT 06511, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7157-9572","authenticated-orcid":true,"given":"Camila","family":"Robles-Oteiza","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Internal Medicine (Section of Medical Oncology), Yale School of Medicine, New Haven, CT 06511, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4662-077X","authenticated-orcid":true,"given":"Antariksh","family":"Tyagi","sequence":"additional","affiliation":[{"name":"Yale Center for Genome Analysis, Yale University, New Haven, CT 06516, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Politi","sequence":"additional","affiliation":[{"name":"Yale Cancer Center, Yale University, New Haven, CT 06511, USA."},{"name":"Departments of Pathology and Internal Medicine (Section of Medical Oncology), Yale School of Medicine, New Haven, CT 06511, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8714-1049","authenticated-orcid":true,"given":"Jesse R.","family":"Zamudio","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5983-1496","authenticated-orcid":true,"given":"Nadya","family":"Dimitrova","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06511, USA."},{"name":"Yale Cancer Center, Yale University, New Haven, CT 06511, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202001951R"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2015.09.006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-021-00353-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2004.07.979"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206928"},{"key":"e_1_3_2_7_2","first-page":"22","article-title":"MALAT1 long non-coding RNA: Functional implications","volume":"6","author":"Arun G.","year":"2020","unstructured":"G. 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