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Deleting the upstream super-enhancer region mainly affected constitutive CD25 expression on DN2\/DN3 thymocytes and T\n            <jats:sub>regs<\/jats:sub>\n            , with these mice developing autoimmune alopecia, whereas deleting an intronic region decreased IL-2\u2013induced CD25 on peripheral T and NK cells. Thus, distinct super-enhancer elements preferentially control constitutive versus inducible expression in a cell type\u2013specific manner. The mediator-1 coactivator colocalized with specific STAT5-binding sites. Moreover, both upstream and intronic regions had extensive chromatin interactions, and deletion of either region altered the super-enhancer structure in mature T cells. These results demonstrate differential functions for distinct super-enhancer elements, thereby indicating previously unknown ways to manipulate CD25 expression in a cell type\u2013specific fashion.\n          <\/jats:p>","DOI":"10.1126\/sciimmunol.adi8217","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T17:58:31Z","timestamp":1699034311000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":16,"title":["Distinct use of super-enhancer elements controls cell type\u2013specific CD25 transcription and function"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1511-9349","authenticated-orcid":true,"given":"Rosanne","family":"Spolski","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Immunology, Immunology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, 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