{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:36:45Z","timestamp":1790840205181,"version":"4.1.0"},"reference-count":80,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"92","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Immunol."],"published-print":{"date-parts":[[2024,9,2]]},"abstract":"<jats:p>\n                    TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    CD8\u03b1\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    intraepithelial lymphocytes (CD8\u03b1\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    \u03b1\u03b2 IELs) are a specialized subset of T cells in the gut epithelium that develop from thymic agonist selected IEL precursors (IELps). The molecular mechanisms underlying the selection and differentiation of this T cell type in the thymus are largely unknown. Here, we found that\n                    <jats:italic>Bcl6<\/jats:italic>\n                    deficiency in \u03b1\u03b2 T cells resulted in the near absence of CD8\u03b1\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    \u03b1\u03b2 IELs. BCL6 was expressed by approximately 50% of CD8\u03b1\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    \u03b1\u03b2 IELs and by the majority of thymic PD1\n                    <jats:sup>+<\/jats:sup>\n                    IELps after agonist selection.\n                    <jats:italic>Bcl6<\/jats:italic>\n                    deficiency blocked early IELp generation in the thymus, and its expression in IELps was induced by thymic TCR signaling in an ERK-dependent manner. As a result of\n                    <jats:italic>Bcl6<\/jats:italic>\n                    deficiency, the precursors of IELps among CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    double-positive thymocytes exhibited increased apoptosis during agonist selection and impaired IELp differentiation and maturation. Together, our results elucidate BCL6 as a crucial transcription factor during the thymic development of CD8\u03b1\u03b1\n                    <jats:sup>+<\/jats:sup>\n                    \u03b1\u03b2 IELs.\n                  <\/jats:p>","DOI":"10.1126\/sciimmunol.adk4348","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T13:58:10Z","timestamp":1707487090000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":15,"title":["BCL6 is required for the thymic development of TCR\u03b1\u03b2\n                    <sup>+<\/sup>\n                    CD8\u03b1\u03b1\n                    <sup>+<\/sup>\n                    intraepithelial lymphocyte lineage"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8246-5949","authenticated-orcid":true,"given":"Qi","family":"Xing","sequence":"first","affiliation":[{"name":"Shanghai Immune Therapy Institute, New Cornerstone Science Laboratory, Shanghai Jiao Tong University School of Medicine-affiliated Renji Hospital, Shanghai 200127, China."},{"name":"Institute for Advanced Interdisciplinary Studies and Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, Peking University, Beijing 100084, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dehui","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyuan","family":"Xie","sequence":"additional","affiliation":[{"name":"Research Unit of Immune Regulation and Immune Diseases of Chinese Academy of Medical Sciences, Shanghai Jiao Tong University School of Medicine-Affiliated Renji Hospital, Shanghai 200127, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2396-8239","authenticated-orcid":true,"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5073-0488","authenticated-orcid":true,"given":"Xiaohu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9059-3078","authenticated-orcid":true,"given":"Xue","family":"Bai","sequence":"additional","affiliation":[{"name":"Institute for Immunology and School of Medicine, Tsinghua University, Beijing 100084, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0084-9130","authenticated-orcid":true,"given":"Chen","family":"Dong","sequence":"additional","affiliation":[{"name":"Shanghai Immune Therapy Institute, New Cornerstone Science Laboratory, Shanghai Jiao Tong University School of Medicine-affiliated Renji Hospital, Shanghai 200127, China."},{"name":"Research Unit of Immune Regulation and Immune Diseases of Chinese Academy of Medical Sciences, Shanghai Jiao Tong University School of Medicine-Affiliated Renji Hospital, Shanghai 200127, China."},{"name":"Westlake University School of Medicine-affiliated Hangzhou First Hospital, Hangzhou 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