{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:48:09Z","timestamp":1792010889555,"version":"4.4.0"},"reference-count":54,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"114","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HL122531"],"award-info":[{"award-number":["R01 HL122531"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HL150520"],"award-info":[{"award-number":["R01 HL150520"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35 GM137430"],"award-info":[{"award-number":["R35 GM137430"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000875","name":"Pew Charitable Trusts","doi-asserted-by":"publisher","award":["00034105"],"award-info":[{"award-number":["00034105"]}],"id":[{"id":"10.13039\/100000875","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pew Charitable Trusts Latin American Fellowship","award":["00034105"],"award-info":[{"award-number":["00034105"]}]}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Immunol."],"published-print":{"date-parts":[[2025,12,19]]},"abstract":"<jats:p>\n                    Neutrophils have vital proinflammatory protective functions, but gene expression changes in neutrophils found in inflamed tissues suggest additional proresolving effects. We identified a neutrophil subset with a distinct phenotype and function that emerges in mouse lungs during resolution of injury. These resolution-phase neutrophils increased expression of Siglec-F (sialic acid\u2013binding Ig-like lectin F),\n                    <jats:italic toggle=\"yes\">Alox15<\/jats:italic>\n                    (12\/15-lipoxygenase), and\n                    <jats:italic toggle=\"yes\">Csf1<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">colony-stimulating factor 1<\/jats:italic>\n                    ). Siglec-F\n                    <jats:sup>+<\/jats:sup>\n                    neutrophils promoted macrophage differentiation and produced specialized proresolving mediators that accelerated injury resolution. Neutrophil depletion hindered lung epithelial catabatic responses, whereas adoptive transfer of Siglec-F\n                    <jats:sup>+<\/jats:sup>\n                    neutrophils accelerated restitution of lung epithelial cells. Transforming growth factor\u2013\u03b2 (TGF-\u03b2) and granulocyte-macrophage colony-stimulating factor (GM-CSF), acting via activator protein-1 (AP-1)\/Jun, promoted expression of Siglec-F in mouse neutrophils and ALOX15 in mouse and human neutrophils. In patients with respiratory failure, ALOX15\n                    <jats:sup>+<\/jats:sup>\n                    neutrophils were present in the bronchoalveolar lavage samples, and their frequency correlated with improved oxygenation. Thus, Siglec-F\n                    <jats:sup>+<\/jats:sup>\n                    ALOX15\n                    <jats:sup>+<\/jats:sup>\n                    proresolving neutrophils contribute to tissue injury responses.\n                  <\/jats:p>","DOI":"10.1126\/sciimmunol.aeb2657","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T19:00:32Z","timestamp":1766170832000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":15,"title":["Siglec-F\n                    <sup>+<\/sup>\n                    neutrophils promote the resolution of acute lung injury through ALOX15 induction"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5319-0210","authenticated-orcid":true,"given":"Luciana P.","family":"Tavares","sequence":"first","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thayse R.","family":"Br\u00fcggemann","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4210-7928","authenticated-orcid":true,"given":"R. Elaine","family":"Cagnina","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0832-9938","authenticated-orcid":true,"given":"Robert","family":"Nshimiyimana","sequence":"additional","affiliation":[{"name":"Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3940-5617","authenticated-orcid":true,"given":"Ana B.","family":"Villase\u00f1or-Altamirano","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael M.","family":"Rezende","sequence":"additional","affiliation":[{"name":"Ann Romney Center for Neurologic Diseases, Department of Neurology, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2250-6075","authenticated-orcid":true,"given":"Toby B.","family":"Lanser","sequence":"additional","affiliation":[{"name":"Ann Romney Center for Neurologic Diseases, Department of Neurology, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2904-4413","authenticated-orcid":true,"given":"Xiaoli","family":"Liu","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3323-2337","authenticated-orcid":true,"given":"Marjorie E.","family":"Bateman","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1114-7118","authenticated-orcid":true,"given":"Nandini","family":"Krishnamoorthy","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7372-3563","authenticated-orcid":true,"given":"Stephanie","family":"Pons","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4173-3628","authenticated-orcid":true,"given":"Melody G.","family":"Duvall","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine Division, Department of Medicine, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8053-0145","authenticated-orcid":true,"given":"Raja-Elie E.","family":"Abdulnour","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care 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