{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T09:24:11Z","timestamp":1784366651429,"version":"3.55.0"},"reference-count":70,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"55","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Signal."],"published-print":{"date-parts":[[2009,1,27]]},"abstract":"<jats:p>An NMR-based assay enables real-time analysis of the GTPase activity of Rheb and of the effect of its GTPase-activating protein TSC2.<\/jats:p>","DOI":"10.1126\/scisignal.2000029","type":"journal-article","created":{"date-parts":[[2009,3,23]],"date-time":"2009-03-23T20:03:20Z","timestamp":1237838600000},"source":"Crossref","is-referenced-by-count":64,"title":["Characterization of the Intrinsic and TSC2-GAP\u2013Regulated GTPase Activity of Rheb by Real-Time NMR"],"prefix":"10.1126","volume":"2","author":[{"given":"Christopher B.","family":"Marshall","sequence":"first","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Ho","sequence":"additional","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Buerger","sequence":"additional","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Plevin","sequence":"additional","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Yao","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiko","family":"Ikura","sequence":"additional","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vuk","family":"Stambolic","sequence":"additional","affiliation":[{"name":"Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9."},{"name":"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)00071-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34012-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306553200"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb996"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00220-X"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb999"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1110003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra055323"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20080281"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi260"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00398.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.887201"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2006.04.003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501253200"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.6.1053"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1617608"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb839"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00568-3"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209882"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00929-2"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205838200"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.02.031"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405659101"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303956200"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.058"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.055"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1256804"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99008-3"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5324.333"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.004023"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90018-A"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/332548a0"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205792"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.18.7965-7975.2004"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6565(98)00025-9"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi970296u"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00121a010"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00477a025"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/312071a0"},{"key":"e_1_3_2_42_2","first-page":"2050","article-title":"Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: The C-terminal domain of GAP is not sufficient for full activity.","volume":"12","author":"Gideon P.","year":"1992","unstructured":"Gideon P., John J., Frech M., Lautwein A., Clark R., Scheffler J. E., Wittinghofer A., Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: The C-terminal domain of GAP is not sufficient for full activity. Mol. Cell. Biol. 12, 2050\u2013 2056 (1992).","journal-title":"Mol. Cell. Biol."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.12.7065"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)37854-2"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphys.2004.06.051"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/39651"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02505"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1086\/302381"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.11.1991"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1086\/316951"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1086\/301705"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1097\/GIM.0b013e31803068c7"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-001-0903-7"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20227"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1998)12:6<408::AID-HUMU7>3.0.CO;2-P"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.30"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2005.127"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)51599-4"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)07002-3"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb17820.x"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)07005-9"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00506-2"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5136-x"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.11.022"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0712095105"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr0503106"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109176200"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702069104"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.20.7687"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00197809"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg520"}],"container-title":["Science Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/scisignal.2000029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T06:55:55Z","timestamp":1705388155000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/scisignal.2000029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,27]]},"references-count":70,"journal-issue":{"issue":"55","published-print":{"date-parts":[[2009,1,27]]}},"alternative-id":["10.1126\/scisignal.2000029"],"URL":"https:\/\/doi.org\/10.1126\/scisignal.2000029","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1157968.618047","asserted-by":"object"}]},"ISSN":["1945-0877","1937-9145"],"issn-type":[{"value":"1945-0877","type":"print"},{"value":"1937-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1,27]]}}}