{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:05:51Z","timestamp":1791925551346,"version":"4.3.4"},"reference-count":67,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"507","funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["award332644"],"award-info":[{"award-number":["award332644"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB1027, BO3643\/3-1"],"award-info":[{"award-number":["SFB1027, BO3643\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["award332643"],"award-info":[{"award-number":["award332643"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["13-21053S"],"award-info":[{"award-number":["13-21053S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["award332635"],"award-info":[{"award-number":["award332635"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P28872"],"award-info":[{"award-number":["P28872"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["award332640"],"award-info":[{"award-number":["award332640"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P28701"],"award-info":[{"award-number":["P28701"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["award332641"],"award-info":[{"award-number":["award332641"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P26067, P27263"],"award-info":[{"award-number":["P26067, P27263"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["award332642"],"award-info":[{"award-number":["award332642"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P28498"],"award-info":[{"award-number":["P28498"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["award332646"],"award-info":[{"award-number":["award332646"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["W1226"],"award-info":[{"award-number":["W1226"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004937","name":"Bundesministerium f\u00fcr Forschung und Technologie","doi-asserted-by":"publisher","award":["award332645"],"award-info":[{"award-number":["award332645"]}],"id":[{"id":"10.13039\/501100004937","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004937","name":"Bundesministerium f\u00fcr Forschung und Technologie","doi-asserted-by":"publisher","award":["HRSM project"],"award-info":[{"award-number":["HRSM project"]}],"id":[{"id":"10.13039\/501100004937","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Signal."],"published-print":{"date-parts":[[2017,11,28]]},"abstract":"<jats:p>Characterization of disease-associated Orai1 mutants reveals the multistep gating mechanism for the CRAC channel.<\/jats:p>","DOI":"10.1126\/scisignal.aao0358","type":"journal-article","created":{"date-parts":[[2017,11,28]],"date-time":"2017-11-28T14:15:28Z","timestamp":1511878528000},"source":"Crossref","is-referenced-by-count":84,"title":["Transmembrane helix connectivity in Orai1 controls two gates for calcium-dependent transcription"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0661-2932","authenticated-orcid":false,"given":"Irene","family":"Frischauf","sequence":"first","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Litvi\u0148ukov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."},{"name":"Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Cardiovascular and Metabolic Sciences, Berlin D-13125, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romana","family":"Schober","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasilina","family":"Zayats","sequence":"additional","affiliation":[{"name":"Center for Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Nov\u00e9 Hrady CZ-373 33, Czech Republic."},{"name":"Center of New Technologies, University of Warsaw, Warsaw 02-097, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbora","family":"Svobodov\u00e1","sequence":"additional","affiliation":[{"name":"Institute for Biophysics, Medical University of Graz, Graz A-8010, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6069-7642","authenticated-orcid":false,"given":"Daniel","family":"Bonhenry","sequence":"additional","affiliation":[{"name":"Center for Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Nov\u00e9 Hrady CZ-373 33, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Lunz","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Cappello","sequence":"additional","affiliation":[{"name":"Molecular Physiology, Institute of Cardiovascular Physiology, University Medical Center, Georg August University G\u00f6ttingen, G\u00f6ttingen, Niedersachsen 37073, Germany."},{"name":"Department of Biophysics, Center for Integrative Physiology and Molecular Medicine, Medical Faculty, Saarland University, Homburg D-66421, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6218-5352","authenticated-orcid":false,"given":"Laura","family":"Tociu","sequence":"additional","affiliation":[{"name":"Center for Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Nov\u00e9 Hrady CZ-373 33, Czech Republic."},{"name":"University of Chicago, Chicago, IL 60637, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Reha","sequence":"additional","affiliation":[{"name":"Center for Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Nov\u00e9 Hrady CZ-373 33, Czech Republic."},{"name":"Faculty of Sciences, University of South Bohemia, Nov\u00e9 Hrady CZ-373 33, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrutha","family":"Stallinger","sequence":"additional","affiliation":[{"name":"Institute for Molecular Biosciences, Karl-Franzens-University Graz, Graz A-8010, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Hochreiter","sequence":"additional","affiliation":[{"name":"Institute for Experimental and Clinical Cell Therapy, Paracelsus Medical University, Salzburg A-5020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Pammer","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4581-4262","authenticated-orcid":false,"given":"Carmen","family":"Butorac","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2583-5939","authenticated-orcid":false,"given":"Martin","family":"Muik","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Groschner","sequence":"additional","affiliation":[{"name":"Institute for Biophysics, Medical University of Graz, Graz A-8010, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Bogeski","sequence":"additional","affiliation":[{"name":"Molecular Physiology, Institute of Cardiovascular Physiology, University Medical Center, Georg August University G\u00f6ttingen, G\u00f6ttingen, Niedersachsen 37073, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8624-7706","authenticated-orcid":false,"given":"R\u00fcdiger H.","family":"Ettrich","sequence":"additional","affiliation":[{"name":"Center for Nanobiology and Structural Biology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Nov\u00e9 Hrady CZ-373 33, Czech Republic."},{"name":"Faculty of Sciences, University of South Bohemia, Nov\u00e9 Hrady CZ-373 33, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3756-4136","authenticated-orcid":false,"given":"Christoph","family":"Romanin","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, JKU Life Science Center, Johannes Kepler University Linz, Linz A-4020, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0896-8887","authenticated-orcid":false,"given":"Rainer","family":"Schindl","sequence":"additional","affiliation":[{"name":"Institute for Biophysics, Medical University of Graz, Graz A-8010, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04702"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-171611"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/306067a0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.08.006"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04147"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07065"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.79"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.02.014"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1842"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3963"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.02.027"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aab1901"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.4161\/chan.24302"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12938"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.04.025"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1103315108"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2008.12.019"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12222"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1113\/JP271141"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.12.009"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1228757"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10752"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14512"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808312200"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1114821108"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.10.003"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2004088"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/369497a0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8235597"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.04.032"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu477"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11252"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13480"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12113"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14129"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C800229200"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3114"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1204592"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14587"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03664"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1174447"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M803101200"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C109.024083"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906781106"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904664106"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6164"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001169107"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909574106"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1316969110"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.501510"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.685289"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.250456"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13725"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.80"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.265884"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.20028551"},{"key":"e_1_3_2_58_2","unstructured":"B. Hille Ion Channels of Excitable Membranes (Sinauer 2001)."},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10104"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0263-7855(97)00009-X"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21787"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2014.05.037"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aad7808"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2011.10.023"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp402839r"},{"key":"e_1_3_2_67_2","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott O.","year":"2010","unstructured":"O. Trott, A. J. Olson, AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J. Comput. Chem. 31, 455\u2013461 (2010).","journal-title":"J. Comput. Chem."},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-987X(19981115)19:14<1639::AID-JCC10>3.0.CO;2-B"}],"container-title":["Science Signaling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1126\/scisignal.aao0358","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/scisignal.aao0358","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T05:59:32Z","timestamp":1705384772000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/scisignal.aao0358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,28]]},"references-count":67,"journal-issue":{"issue":"507","published-print":{"date-parts":[[2017,11,28]]}},"alternative-id":["10.1126\/scisignal.aao0358"],"URL":"https:\/\/doi.org\/10.1126\/scisignal.aao0358","relation":{},"ISSN":["1945-0877","1937-9145"],"issn-type":[{"value":"1945-0877","type":"print"},{"value":"1937-9145","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,28]]},"article-number":"eaao0358"}}