{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:19:02Z","timestamp":1790371142575,"version":"4.1.0"},"reference-count":77,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"752","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Signal."],"published-print":{"date-parts":[[2022,9,20]]},"abstract":"<jats:p>\n            Goblet cells in the small intestinal crypts contain large numbers of mucin granules that are rapidly discharged to clean bacteria from the crypt. Because acetylcholine released by neuronal and nonneuronal cells controls many aspects of intestinal epithelial function, we used tissue explants and organoids to investigate the response of the small intestinal crypt to cholinergic stimulation. The activation of muscarinic acetylcholine receptors initiated a coordinated and rapid emptying of crypt goblet cells that flushed the crypt contents into the intestinal lumen. Cholinergic stimulation induced an expansion of the granule contents followed by intracellular rupture of the mucin granules. The mucus expanded intracellularly before the rupture of the goblet cell apical membrane and continued to expand after its release into the crypt lumen. The goblet cells recovered from membrane rupture and replenished their stores of mucin granules. Mucus secretion from the goblet cells depended on Ca\n            <jats:sup>2+<\/jats:sup>\n            signaling and the expansion of the mucus in the crypt depended on gap junctions and on ion and water transport by enterocytes adjacent to the goblet cells. This distinctive mode of mucus secretion, which we refer to as \u201cexpanding secretion,\u201d efficiently cleans the small intestine crypt through coordinated mucus, ion, and fluid secretion by goblet cells and enterocytes.\n          <\/jats:p>","DOI":"10.1126\/scisignal.abl5848","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T17:58:14Z","timestamp":1663696694000},"source":"Crossref","is-referenced-by-count":57,"title":["Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport"],"prefix":"10.1126","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9062-3882","authenticated-orcid":true,"given":"Brendan","family":"Dolan","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 405 30 Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3233-043X","authenticated-orcid":true,"given":"Anna","family":"Ermund","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 405 30 Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0521-3473","authenticated-orcid":true,"given":"Beatriz","family":"Martinez-Abad","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 405 30 Gothenburg, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4237-6677","authenticated-orcid":true,"given":"Malin E. 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