{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:11:12Z","timestamp":1792033872868,"version":"4.4.0"},"reference-count":76,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"867","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Signal."],"published-print":{"date-parts":[[2024,12,17]]},"abstract":"<jats:p>\n            Bile acids (BAs) affect the growth of potentially pathogenic commensals, including those from the Enterobacteriaceae family, which are frequently overrepresented in inflammatory bowel disease (IBD). BAs are normally reabsorbed in the ileum for recycling and are often increased in the colonic lumina of patients with IBD, including those with Crohn\u2019s disease (CD). Here, we investigated the influence of BAs on gut colonization by Enterobacteriaceae. We found increased abundance of Enterobacteriaceae in the colonic mucosae of patients with CD with a concomitant decrease in the transporters that resorb BAs in the ileum. The increase in Enterobacteriaceae colonization was greater in the colons of patients who had undergone terminal ileum resection compared with those with intact ileum, leading us to hypothesize that BAs promote intestinal colonization by Enterobacteriaceae. Exposure of human colonic epithelial cell lines to BAs reduced mitochondrial respiration, increased oxygen availability, and enhanced the epithelial adherence of several Enterobacteriaceae members. In a publicly available human dataset, mucosal Enterobacteriaceae was negatively associated with the expression of genes related to mitochondrial function. In a murine model, increased intestinal BA availability enhanced colonization by\n            <jats:italic>Escherichia coli<\/jats:italic>\n            in a manner that depended on bacterial respiration. Together, our findings demonstrate that BAs reduce mitochondrial respiration in the colon, leading to an increase in oxygen availability that facilitates Enterobacteriaceae colonization. This identification of BAs as facilitators of host-commensal interactions may be relevant to multiple intestinal diseases.\n          <\/jats:p>","DOI":"10.1126\/scisignal.adl1786","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T18:58:54Z","timestamp":1734461934000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":12,"title":["Bile acid\u2013induced metabolic changes in the colon promote Enterobacteriaceae expansion and associate with dysbiosis in Crohn\u2019s disease"],"prefix":"10.1126","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7889-6757","authenticated-orcid":true,"given":"Ravi","family":"Holani","sequence":"first","affiliation":[{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada."},{"name":"Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, 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