{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T08:01:51Z","timestamp":1786780911798,"version":"build-2736575974"},"reference-count":49,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"593","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI138978"],"award-info":[{"award-number":["R01AI138978"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54EB007958"],"award-info":[{"award-number":["U54EB007958"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R61AI154628"],"award-info":[{"award-number":["R61AI154628"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI138978"],"award-info":[{"award-number":["R01AI138978"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Transl. Med."],"published-print":{"date-parts":[[2021,5,12]]},"abstract":"<jats:p>A portable magnetofluidic platform integrates nucleic acid purification and PCR to rapidly detect the pathogen causing gonorrhea in clinical samples.<\/jats:p>","DOI":"10.1126\/scitranslmed.abf6356","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T19:10:32Z","timestamp":1620846632000},"source":"Crossref","is-referenced-by-count":65,"title":["A portable magnetofluidic platform for detecting sexually transmitted infections and antimicrobial susceptibility"],"prefix":"10.1126","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3997-9669","authenticated-orcid":true,"given":"Alexander Y.","family":"Trick","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan H.","family":"Melendez","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6970-4370","authenticated-orcid":true,"given":"Fan-En","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0648-2456","authenticated-orcid":true,"given":"Liben","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6741-1512","authenticated-orcid":true,"given":"Annet","family":"Onzia","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aidah","family":"Zawedde","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"Nakku-Joloba","sequence":"additional","affiliation":[{"name":"School of Public Health, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9606-218X","authenticated-orcid":true,"given":"Peter","family":"Kyambadde","sequence":"additional","affiliation":[{"name":"AIDS Control Program, Division of Sexually Transmitted Infections, Ministry of Health, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0587-8181","authenticated-orcid":true,"given":"Emmanuel","family":"Mande","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Matovu","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2008-9896","authenticated-orcid":true,"given":"Maxine","family":"Atuheirwe","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5270-3539","authenticated-orcid":true,"given":"Richard","family":"Kwizera","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1713-5107","authenticated-orcid":true,"given":"Elizabeth A.","family":"Gilliams","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA."},{"name":"Sexual Health Clinics, Baltimore City Health Department, Baltimore, MD 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1616-8014","authenticated-orcid":true,"given":"Yu-Hsiang","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1021-3195","authenticated-orcid":true,"given":"Charlotte A.","family":"Gaydos","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukari C.","family":"Manabe","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA."},{"name":"Infectious Diseases Institute, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1277-819X","authenticated-orcid":true,"given":"Matthew M.","family":"Hamill","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA."},{"name":"Sexual Health Clinics, Baltimore City Health Department, Baltimore, MD 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3540-9354","authenticated-orcid":true,"given":"Tza-Huei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA."},{"name":"Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA."},{"name":"Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA."},{"name":"Institute for NanoBiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/pop.2017.0132"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.18.228486"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"M. Unemo D. Golparian D. W. Eyre Antimicrobial resistance in Neisseria gonorrhoeae and treatment of gonorrhea in Neisseria gonorrhoeae Methods in Molecular Biology M. Christodoulides Ed. (Humana 2019) vol. 1997 pp. 37\u201358.","DOI":"10.1007\/978-1-4939-9496-0_3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00010-14"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002366"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2020.1778467"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2196\/17009"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005611"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix450"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2016-053072"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2014.871495"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2017-053443"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000865"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000230"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30310-9"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2014.880651"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-014-1060-2"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1039\/C7LC00025A"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200503624"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201300383"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2013.06.011"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04781-8"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28124-3"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000154495.24519.bf"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3281-3283.2004"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000591"},{"key":"e_1_3_2_28_2","first-page":"76","article-title":"Rapid cycle DNA amplification: Time and temperature optimization","volume":"10","author":"Wittwer C. T.","year":"1991","unstructured":"C. T. Wittwer, D. J. Garling, Rapid cycle DNA amplification: Time and temperature optimization. Biotechniques 10, 76\u201383 (1991).","journal-title":"Biotechniques"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2014.228304"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2019.100081"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl416"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1an15365j"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2011.06.017"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000755"},{"key":"e_1_3_2_35_2","first-page":"332","article-title":"Update to CDC\u2019s Sexually Transmitted Diseases Treatment Guidelines, 2006: Fluoroquinolones no longer recommended for treatment of gonococcal infections","volume":"56","author":"Centers for Disease Control and Prevention (CDC)","year":"2007","unstructured":"Centers for Disease Control and Prevention (CDC), Update to CDC\u2019s Sexually Transmitted Diseases Treatment Guidelines, 2006: Fluoroquinolones no longer recommended for treatment of gonococcal infections. MMWR Morb. Mortal. Wkly Rep. 56, 332\u2013336 (2007).","journal-title":"MMWR Morb. Mortal. Wkly Rep."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss6507a1"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000758"},{"key":"e_1_3_2_38_2","first-page":"1268","article-title":"Implementation of a rapid genotypic assay to promote targeted ciprofloxacin therapy of Neisseria gonorrhoeae in a large health system","volume":"64","author":"Allan-Blitz L.-T.","year":"2016","unstructured":"L.-T. Allan-Blitz, R. M. Humphries, P. Hemarajata, A. Bhatti, M. W. Pandori, M. J. Siedner, J. D. Klausner, Implementation of a rapid genotypic assay to promote targeted ciprofloxacin therapy of Neisseria gonorrhoeae in a large health system. Clin. Infect. Dis. 64, 1268\u20131270 (2016).","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens8040189"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6950a6"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.12.007"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5653-5659.2005"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268811002883"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01624-10"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv366"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01019-10"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aal3693"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-018-0286-y"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0179"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics7030077"}],"container-title":["Science Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1126\/scitranslmed.abf6356","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/scitranslmed.abf6356","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T16:56:51Z","timestamp":1705424211000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/scitranslmed.abf6356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,12]]},"references-count":49,"journal-issue":{"issue":"593","published-print":{"date-parts":[[2021,5,12]]}},"alternative-id":["10.1126\/scitranslmed.abf6356"],"URL":"https:\/\/doi.org\/10.1126\/scitranslmed.abf6356","relation":{},"ISSN":["1946-6234","1946-6242"],"issn-type":[{"value":"1946-6234","type":"print"},{"value":"1946-6242","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,12]]},"article-number":"eabf6356"}}