{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T22:00:51Z","timestamp":1793656851644,"version":"4.5.1"},"reference-count":59,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"630","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Transl. Med."],"published-print":{"date-parts":[[2022,2,2]]},"abstract":"<jats:p>\n                    Skin is composed of diverse cell populations that cooperatively maintain homeostasis. Up-regulation of the nuclear factor \u03baB (NF-\u03baB) pathway may lead to the development of chronic inflammatory disorders of the skin, but its role during the early events remains unclear. Through analysis of single-cell RNA sequencing data via iterative random forest leave one out prediction, an explainable artificial intelligence method, we identified an immunoregulatory role for a unique paired related homeobox-1 (Prx1)\n                    <jats:sup>+<\/jats:sup>\n                    fibroblast subpopulation. Disruption of\n                    <jats:italic>Ikkb<\/jats:italic>\n                    \u2013NF-\u03baB under homeostatic conditions in these fibroblasts paradoxically induced skin inflammation due to the overexpression of C-C motif chemokine ligand 11 (CCL11; or eotaxin-1) characterized by eosinophil infiltration and a subsequent T\n                    <jats:sub>H<\/jats:sub>\n                    2 immune response. Because the inflammatory phenotype resembled that seen in human atopic dermatitis (AD), we examined human AD skin samples and found that human AD fibroblasts also overexpressed CCL11 and that perturbation of\n                    <jats:italic>Ikkb<\/jats:italic>\n                    \u2013NF-\u03baB in primary human dermal fibroblasts up-regulated CCL11. Monoclonal antibody treatment against CCL11 was effective in reducing the eosinophilia and T\n                    <jats:sub>H<\/jats:sub>\n                    2 inflammation in a mouse model. Together, the murine model and human AD specimens point to dysregulated Prx1\n                    <jats:sup>+<\/jats:sup>\n                    fibroblasts as a previously unrecognized etiologic factor that may contribute to the pathogenesis of AD and suggest that targeting CCL11 may be a way to treat AD-like skin lesions.\n                  <\/jats:p>","DOI":"10.1126\/scitranslmed.abj0324","type":"journal-article","created":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T13:53:15Z","timestamp":1643809995000},"source":"Crossref","is-referenced-by-count":81,"title":["NF-\u03baB perturbation reveals unique immunomodulatory functions in Prx1\n                    <sup>+<\/sup>\n                    fibroblasts that promote development of atopic dermatitis"],"prefix":"10.1126","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2967-8846","authenticated-orcid":true,"given":"Kang I.","family":"Ko","sequence":"first","affiliation":[{"name":"Department of Periodontics, School of 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