{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T17:36:51Z","timestamp":1782841011132,"version":"3.54.5"},"reference-count":33,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"683","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Transl. Med."],"published-print":{"date-parts":[[2023,2,15]]},"abstract":"<jats:p>NDV-HXP-S is a recombinant Newcastle disease virus\u2013based vaccine against SARS-CoV-2, which expresses an optimized (HexaPro) spike protein on its surface. The vaccine can be produced in embryonated chicken eggs using the same process as that used for the production of the vast majority of influenza virus vaccines. Here, we performed a secondary analysis of the antibody responses after vaccination with inactivated NDV-HXP-S in a phase 1 clinical study in Thailand. The SARS-CoV-2 neutralizing and spike protein binding activity of NDV-HXP-S postvaccination serum samples was compared to that of samples from mRNA BNT162b2 (Pfizer) vaccinees. Neutralizing activity of sera from NDV-HXP-S vaccinees was comparable to that of BNT162b2 vaccinees, whereas spike protein binding activity of the NDV-HXP-S vaccinee samples was lower than that of sera obtained from mRNA vaccinees. This led us to calculate ratios between binding and neutralizing antibody titers. Samples from NDV-HXP-S vaccinees had binding to neutralizing activity ratios that were lower than those of BNT162b2 sera, suggesting that NDV-HXP-S vaccination elicits a high proportion of neutralizing antibodies and low non-neutralizing antibody titers. Further analysis showed that, in contrast to mRNA vaccination, which induces strong antibody titers to the receptor binding domain (RBD), the N-terminal domain, and the S2 domain, NDV-HXP-S vaccination induced an RBD-focused antibody response with little reactivity to S2. This finding may explain the high proportion of neutralizing antibodies. In conclusion, vaccination with inactivated NDV-HXP-S induces a high proportion of neutralizing antibodies and absolute neutralizing antibody titers that are comparable to those elicited by mRNA vaccination.<\/jats:p>","DOI":"10.1126\/scitranslmed.abo2847","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T18:58:40Z","timestamp":1676487520000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":22,"title":["An inactivated NDV-HXP-S COVID-19 vaccine elicits a higher proportion of neutralizing antibodies in humans than mRNA vaccination"],"prefix":"10.1126","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9039-9275","authenticated-orcid":true,"given":"Juan Manuel","family":"Carre\u00f1o","sequence":"first","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9110-8217","authenticated-orcid":true,"given":"Ariel","family":"Raskin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5848-2741","authenticated-orcid":true,"given":"Gagandeep","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2733-4687","authenticated-orcid":true,"given":"Johnstone","family":"Tcheou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3987-3429","authenticated-orcid":true,"given":"Hisaaki","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0739-1270","authenticated-orcid":true,"given":"Charles","family":"Gleason","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Komal","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4195-4858","authenticated-orcid":true,"given":"Vladimir","family":"Vigdorovich","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, WA 98109, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Dambrauskas","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, WA 98109, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sneh Lata","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Centers for Childhood Infections and Vaccines, Children\u2019s Healthcare of Atlanta, Emory University, Atlanta, GA 30329, USA."},{"name":"Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30329, 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Noah","family":"Sather","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children\u2019s Research Institute, Seattle, WA 98109, USA."},{"name":"Department of Pediatrics, University of Washington, Seattle, WA 98109, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rama","family":"Raghunandan","sequence":"additional","affiliation":[{"name":"PATH, 2201 Westlake Avenue, Suite 200, Seattle, WA 98121, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ponthip","family":"Wirachwong","sequence":"additional","affiliation":[{"name":"Government Pharmaceutical Organization, 75\/1 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8236-8954","authenticated-orcid":true,"given":"Sant","family":"Muangnoicharoen","sequence":"additional","affiliation":[{"name":"Vaccine Trial Centre Faculty of Tropical Medicine, 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Pl, New\u00a0York, NY 10029, USA."},{"name":"Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."},{"name":"Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."},{"name":"Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6416-5096","authenticated-orcid":true,"given":"Viviana","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."},{"name":"Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."},{"name":"Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."},{"name":"Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4121-776X","authenticated-orcid":true,"given":"Florian","family":"Krammer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."},{"name":"Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, 1 Gustave L.\u00a0Levy Pl, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2798-3"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26499-y"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd0826"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.12.018"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines8040771"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.103132"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.791764"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01908-21"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","unstructured":"P. Pitisuttithum V.\u00a0Luvira S.\u00a0Lawpoolsri S.\u00a0Muangnoicharoen S.\u00a0Kamolratanakul C.\u00a0Sivakorn P.\u00a0Narakorn S.\u00a0Surichan S.\u00a0Prangpratanporn S.\u00a0Puksuriwong S.\u00a0Lamola L.\u00a0D.\u00a0Mercer R.\u00a0Raghunandan W.\u00a0Sun Y.\u00a0Liu J.\u00a0M.\u00a0Carre\u00f1o R.\u00a0Scharf W.\u00a0Phumratanaprapin F.\u00a0Amanat L.\u00a0Gagnon C.\u00a0L.\u00a0Hsieh R.\u00a0Kaweepornpoj S.\u00a0Khan M.\u00a0Lal S.\u00a0McCroskery J.\u00a0McLellan I.\u00a0Mena M.\u00a0Meseck B.\u00a0Phonrat Y.\u00a0Sabmee R.\u00a0Singchareon S.\u00a0Slamanig N.\u00a0Suthepakul J.\u00a0Tcheou N.\u00a0Thantamnu S.\u00a0Theerasurakarn S.\u00a0Tran T.\u00a0Vilasmongkolchai J.\u00a0A.\u00a0White A.\u00a0Garcia-Sastre P.\u00a0Palese F.\u00a0Krammer K.\u00a0Poopipatpol P.\u00a0Wirachwong R.\u00a0Hjorth B.\u00a0L.\u00a0Innis Safety and immunogenicity of an inactivated recombinant Newcastle disease virus vaccine expressing SARS-CoV-2 spike: Interim results of a randomised placebo-controlled phase 1\/2 trial. medRxiv 2021.09.17.21263758 [ Preprint ]. 20 September 2021. https:\/\/doi.org\/10.1101\/2021.09.17.21263758.","DOI":"10.1016\/j.eclinm.2022.101323"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.06.005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2021.03.005"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109353"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.03.028"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03207-w"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04060-7"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj3321"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103626"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04399-5"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.05.063"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2101667"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6445-1_2"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102937"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.100"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.10.011"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abm3425"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01377-8"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2022.101323"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0913-5"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2021.100253"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.116"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2021.03.009"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2107799"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006862"}],"container-title":["Science Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/scitranslmed.abo2847","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T17:55:30Z","timestamp":1705427730000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/scitranslmed.abo2847"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,15]]},"references-count":33,"journal-issue":{"issue":"683","published-print":{"date-parts":[[2023,2,15]]}},"alternative-id":["10.1126\/scitranslmed.abo2847"],"URL":"https:\/\/doi.org\/10.1126\/scitranslmed.abo2847","relation":{},"ISSN":["1946-6234","1946-6242"],"issn-type":[{"value":"1946-6234","type":"print"},{"value":"1946-6242","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,15]]},"assertion":[{"value":"2022-01-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eabo2847"}}