{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:07:39Z","timestamp":1790842059274,"version":"4.1.0"},"reference-count":44,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"685","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Transl. Med."],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:p>\n            Duchenne muscular dystrophy (DMD) is a severe and progressive myopathy leading to motor and cardiorespiratory impairment. We analyzed samples from patients with DMD and a preclinical rat model of severe DMD and determined that compromised repair capacity of muscle stem cells in DMD is associated with early and progressive muscle stem cell senescence. We also found that extraocular muscles (EOMs), which are spared by the disease in patients, contain muscle stem cells with long-lasting regenerative potential. Using single-cell transcriptomics analysis of muscles from a rat model of DMD, we identified the gene encoding thyroid-stimulating hormone receptor (\n            <jats:italic>Tshr<\/jats:italic>\n            ) as highly expressed in EOM stem cells. Further, TSHR activity was involved in preventing senescence. Forskolin, which activates signaling downstream of TSHR, was found to reduce senescence of skeletal muscle stem cells, increase stem cell regenerative potential, and promote myogenesis, thereby improving muscle function in DMD rats. These findings indicate that stimulation of adenylyl cyclase leads to muscle repair in DMD, potentially providing a therapeutic approach for patients with the disease.\n          <\/jats:p>","DOI":"10.1126\/scitranslmed.add5275","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T18:58:10Z","timestamp":1677697090000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":45,"title":["Thyroid-stimulating hormone receptor signaling restores skeletal muscle stem cell regeneration in rats with muscular dystrophy"],"prefix":"10.1126","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8639-8088","authenticated-orcid":true,"given":"Valentina","family":"Taglietti","sequence":"first","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaouthar","family":"Kefi","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Rivera","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriane","family":"Bergiers","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9115-718X","authenticated-orcid":true,"given":"Nastasia","family":"Cardone","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny","family":"Coulpier","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stamatia","family":"Gioftsidi","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernadette","family":"Drayton-Libotte","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0258-4738","authenticated-orcid":true,"given":"Cyrielle","family":"Hou","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0182-2052","authenticated-orcid":true,"given":"Fran\u00e7ois-J\u00e9r\u00f4me","family":"Authier","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."},{"name":"AP-HP, H\u00f4pital Mondor, FHU SENEC, Service d\u2019histologie, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6258-7594","authenticated-orcid":true,"given":"France","family":"Pietri-Rouxel","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, UMRS974, Center for Research in Myology, F-75013, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3353-8117","authenticated-orcid":true,"given":"Matthieu","family":"Robert","sequence":"additional","affiliation":[{"name":"Borelli centre, UMR 9010, CNRS - SSA - ENS Paris Saclay - Universit\u00e9 Paris Cit\u00e9, F-75016, Paris, France."},{"name":"Ophthalmology Department, Necker Enfants Malades University Hospital, AP-HP, F-75015, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3563-5482","authenticated-orcid":true,"given":"Dominique","family":"Bremond-Gignac","sequence":"additional","affiliation":[{"name":"Ophthalmology Department, Necker Enfants Malades University Hospital, AP-HP, F-75015, Paris, France."},{"name":"INSERM, UMRS1138, Team 17, Sorbonne Paris Cit\u00e9 University, Centre de Recherche des Cordeliers, F-75006, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3426-2901","authenticated-orcid":true,"given":"Claudio","family":"Bruno","sequence":"additional","affiliation":[{"name":"Center of Translational and Experimental Myology, IRCCS Gaslini Institute, DINOGMI, University of Genova, 16147, Genova, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9027-343X","authenticated-orcid":true,"given":"Chiara","family":"Fiorillo","sequence":"additional","affiliation":[{"name":"Center of Translational and Experimental Myology, IRCCS Gaslini Institute, DINOGMI, University of Genova, 16147, Genova, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edoardo","family":"Malfatti","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."},{"name":"AP-HP, H\u00f4pital Mondor, FHU SENEC, Service d\u2019histologie, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8239-3482","authenticated-orcid":true,"given":"Peggy","family":"Lafuste","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8573-8335","authenticated-orcid":true,"given":"Laurent","family":"Tiret","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."},{"name":"\u00c9cole nationale v\u00e9t\u00e9rinaire d\u2019Alfort, IMRB, F-94700, Maisons-Alfort, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1270-1472","authenticated-orcid":true,"given":"Fr\u00e9d\u00e9ric","family":"Relaix","sequence":"additional","affiliation":[{"name":"Univ Paris-Est Cr\u00e9teil, INSERM, U955 IMRB, F-94010 Cr\u00e9teil, France."},{"name":"AP-HP, H\u00f4pital Mondor, FHU SENEC, Service d\u2019histologie, F-94010 Cr\u00e9teil, France."},{"name":"\u00c9cole nationale v\u00e9t\u00e9rinaire d\u2019Alfort, IMRB, F-94700, Maisons-Alfort, France."},{"name":"EFS, IMRB, F-94010, Creteil, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/323646a0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c140048"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20760-6"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/jpm8040041"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-022-01355-2"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410320418"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.2.467"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.02.001"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3823"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100871"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202000111RR"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2009.00481.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904506106"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.19.6.0352"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ando.2011.03.007"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9071730"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ando.2011.03.006"},{"key":"e_1_3_3_19_2","first-page":"5","article-title":"Forskolin and derivatives as tools for studying the role of cAMP","volume":"67","author":"Alasbahi R. 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