{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T22:27:06Z","timestamp":1794090426754,"version":"4.8.0"},"reference-count":90,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"757","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Transl. Med."],"published-print":{"date-parts":[[2024,7,24]]},"abstract":"<jats:p>\n            Messenger RNA (mRNA) vaccines were pivotal in reducing severe acute respiratory syndrome 2 (SARS-CoV-2) infection burden, yet they have not demonstrated robust durability, especially in older adults. Here, we describe a molecular adjuvant comprising a lipid nanoparticle (LNP)\u2013encapsulated mRNA encoding interleukin-12p70 (IL-12p70). The bioactive adjuvant was engineered with a multiorgan protection (MOP) sequence to restrict transcript expression to the intramuscular injection site. Admixing\n            <jats:italic>IL-12\u2013MOP<\/jats:italic>\n            (CTX-1796) with the BNT162b2 SARS-CoV-2 vaccine increased spike protein\u2013specific immune responses in mice. Specifically, the benefits of\n            <jats:italic>IL-12\u2013MOP<\/jats:italic>\n            adjuvantation included amplified humoral and cellular immunity and increased immune durability for 1 year after vaccination in mice. An additional benefit included the restoration of immunity in aged mice to amounts comparable to those achieved in young adult animals, alongside amplification with a single immunization. Associated enhanced dendritic cell and germinal center responses were observed. Together, these data demonstrate that an LNP-encapsulated\n            <jats:italic>IL-12\u2013MOP<\/jats:italic>\n            mRNA-encoded adjuvant can amplify immunogenicity independent of age, demonstrating translational potential to benefit vulnerable populations.\n          <\/jats:p>","DOI":"10.1126\/scitranslmed.adm8451","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T17:58:36Z","timestamp":1721843916000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":46,"title":["Adjuvantation of a SARS-CoV-2 mRNA vaccine with controlled tissue-specific expression of an mRNA encoding IL-12p70"],"prefix":"10.1126","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3184-8285","authenticated-orcid":true,"given":"Byron","family":"Brook","sequence":"first","affiliation":[{"name":"Precision Vaccines Program, Department of Pediatrics, Boston Children\u2019s Hospital, Boston, MA 02115, 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