{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T19:27:24Z","timestamp":1793388444005,"version":"4.5.0"},"reference-count":78,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"761","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Transl. Med."],"published-print":{"date-parts":[[2024,8,21]]},"abstract":"<jats:p>\n            Neuroinflammation plays a key role in exacerbating dopaminergic neuron (DAN) loss in Parkinson\u2019s disease (PD). However, it remains unresolved how to effectively normalize this immune response given the complex interplay between the innate and adaptive immune responses occurring within a scarcely accessible organ like the brain. In this study, we uncovered a consistent correlation between neuroinflammation, brain parenchymal lymphocytes, and DAN loss among several commonly used mouse models of PD generated by a variety of pathological triggers. We validated a viral therapeutic approach for the microglia-specific expression of interleukin 10 (IL-10) to selectively mitigate the excessive inflammatory response. We found that this approach induced a local nigral IL-10 release that alleviated DAN loss in mice overexpressing the human\n            <jats:italic>SNCA<\/jats:italic>\n            gene in the substantia nigra. Single-cell transcriptomics revealed that IL-10 induced the emergence of a molecularly distinct microglial cell state, enriched in markers of cell activation with enhanced expression of prophagocytic pathways. IL-10 promoted microglial phagocytotic and clearance activities in vitro and reduced \u03b1SYN aggregate burden in the nigral area in mice overexpressing\n            <jats:italic>SNCA<\/jats:italic>\n            . Furthermore, IL-10 stimulated the differentiation of CD4\n            <jats:sup>+<\/jats:sup>\n            T lymphocytes into active T regulatory cells and promoted inhibitory characteristics in CD8\n            <jats:sup>+<\/jats:sup>\n            T cells. In summary, our results show that local and microglia-specific\n            <jats:italic>IL-10<\/jats:italic>\n            transduction elicited strong immunomodulation in the nigral tissue with enhanced suppression of lymphocyte toxicity that was associated with DAN survival. These results offer insights into the therapeutic benefits of IL-10 and showcase a promising gene delivery approach that could minimize undesired side effects.\n          <\/jats:p>","DOI":"10.1126\/scitranslmed.adm8563","type":"journal-article","created":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T22:26:29Z","timestamp":1724279189000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":71,"title":["Microglia-specific\n            <i>IL-10<\/i>\n            gene delivery inhibits neuroinflammation and neurodegeneration in a mouse model of Parkinson\u2019s disease"],"prefix":"10.1126","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3561-9113","authenticated-orcid":true,"given":"Simone","family":"Bido","sequence":"first","affiliation":[{"name":"Stem Cell and Neurogenesis Unit, Division of Neuroscience, IRCCS San Raffaele Scientific Institute, 20132 Milan, 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