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We used two mouse models of type I diabetes that replicate early features of human retinal vascular pathology. In both the streptozotocin (STZ) model, where hypoinsulinemia is chemically induced, and in the Ins2Akita model, which develops it spontaneously because of a mutation in the insulin gene, we observed early induction of the secreted glycoprotein gene leucine-rich \u03b1-2-glycoprotein 1 (\n                    <jats:italic toggle=\"yes\">Lrg1<\/jats:italic>\n                    ). Using the Ins2Akita mice, we showed that\n                    <jats:italic toggle=\"yes\">Lrg1<\/jats:italic>\n                    induction preceded that of vascular endothelial growth factor A (\n                    <jats:italic toggle=\"yes\">Vegfa<\/jats:italic>\n                    ). LRG1 initiated retinal microvascular dysfunction by modifying transforming growth factor\u2013\u03b2 (TGF\u03b2) signaling in pericytes, driving transdifferentiation to a more contractile fibrotic phenotype, resulting in narrower capillaries and thickened basement membrane. Using computational modeling, we showed that these early vascular changes impaired retinal blood flow and oxygen delivery, consistent with a defect in visual transduction observed in both models. This early retinal phenotype could be rescued by\n                    <jats:italic toggle=\"yes\">Lrg1<\/jats:italic>\n                    knockout or by treatment with an LRG1 function\u2013blocking antibody in both the STZ and Ins2Akita mice. These results demonstrate that LRG1 is a driver of vascular dysfunction that contributes to the onset of DR and presents itself as a potential preemptive therapeutic target.\n                  <\/jats:p>","DOI":"10.1126\/scitranslmed.adn6047","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:59:08Z","timestamp":1761155948000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":5,"title":["Leucine-rich \u03b1-2-glycoprotein 1 initiates the onset of diabetic retinopathy in mice"],"prefix":"10.1126","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6163-4590","authenticated-orcid":true,"given":"Giulia","family":"De Rossi","sequence":"first","affiliation":[{"name":"Institute of Ophthalmology, University College London, EC1V 9EL London, 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